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Our Abpromise guarantee covers the use of ab28340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 24466060|
|WB||1/1000 - 1/5000. Detects a band of approximately 110-120 kDa (predicted molecular weight: 88 kDa). 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 for chemiluminescent substrates. Bands run high because of post-translational modifications and reduction. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.|
|IHC-P||Use at an assay dependent concentration.|
ab28340 staining CD26 - Spacer region in rat epididymis tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent paraformaldehyde fixation before heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 and then blocking with 3% hydrogen peroxide in TBS for 10 minutes at 25°C was performed in order to block endogenous peroxidase activity. The primary antibody was used diluted in 1/400 and it was incubated with sample for 16 hours at 4°C in a dilution buffer containing TBS, 0.3% Triton X-100, 0.1 % BSA. A Biotin conjugated goat polyclonal to rabbit IgG was used as secondary antibody at 1/200 dilution.
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