概述

  • 产品名称Anti-CD26抗体
    参阅全部 CD26 一抗
  • 描述
    兔多克隆抗体to CD26
  • 特异性Does not recognize other DPP family members.
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Rhesus monkey
  • 免疫原

    Synthetic peptide corresponding to Human CD26.
    (Peptide available as ab41394)

性能

应用

Our Abpromise guarantee covers the use of ab28340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 24466060
WB 1/1000 - 1/5000. Detects a band of approximately 110-120 kDa (predicted molecular weight: 88 kDa). 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 for chemiluminescent substrates. Bands run high because of post-translational modifications and reduction. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.
  • 组织特异性Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.
  • 序列相似性Belongs to the peptidase S9B family. DPPIV subfamily.
  • 结构域The extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity.
  • 翻译后修饰The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
    N- and O-Glycosylated.
    Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation.
  • 细胞定位Cell membrane. Apical cell membrane. Cell projection > invadopodium membrane. Cell projection > lamellipodium membrane. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface and Secreted. Detected in the serum and the seminal fluid.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD26 antigen antibody
    • ADA-binding protein antibody
    • ADABP antibody
    • ADCP 2 antibody
    • ADCP-2 antibody
    • ADCP2 antibody
    • Adenosine deaminase complexing protein 2 antibody
    • CD 26 antibody
    • CD26 antibody
    • CD26 antigen 3 antibody
    • Dipeptidyl peptidase 4 antibody
    • Dipeptidyl peptidase 4 soluble form antibody
    • Dipeptidyl peptidase IV antibody
    • Dipeptidyl peptidase IV membrane form antibody
    • Dipeptidyl peptidase IV soluble form antibody
    • Dipeptidyl peptidase, intestinal antibody
    • Dipeptidylpeptidase 4 antibody
    • Dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2) antibody
    • Dipeptidylpeptidase IV antibody
    • DPP 4 antibody
    • DPP IV antibody
    • DPP IV estoenzyme antibody
    • DPP4 antibody
    • DPP4_HUMAN antibody
    • DPPIV antibody
    • Intestinal dipeptidyl peptidase antibody
    • T cell activation antigen CD26 antibody
    • T-cell activation antigen CD26 antibody
    • TP 103 antibody
    • TP103 antibody
    see all

Anti-CD26 antibody 图像

  • All lanes : Anti-CD26 antibody (ab28340) at 1/1000 dilution

    Lane 1 : DPP-4 at 0.08 µg
    Lane 2 : DPP-4 at 0.04 µg
    Lane 3 : DPP-4 at 0.02 µg
    Lane 4 : DPP-4 at 0.01 µg


    Predicted band size : 88 kDa
    Observed band size : 115 kDa (why is the actual band size different from the predicted?)
  • ab28340 staining CD26 - Spacer region in rat epididymis tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent paraformaldehyde fixation before heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 and then blocking with 3% hydrogen peroxide in TBS for 10 minutes at 25°C was performed in order to block endogenous peroxidase activity. The primary antibody was used diluted in 1/400 and it was incubated with sample for 16 hours at 4°C in a dilution buffer containing TBS, 0.3% Triton X-100, 0.1 % BSA. A Biotin conjugated goat polyclonal to rabbit IgG was used as secondary antibody at 1/200 dilution.

    See Abreview

Anti-CD26 antibody (ab28340)参考文献

This product has been referenced in:
  • Gieseck RL  et al. Maturation of induced pluripotent stem cell derived hepatocytes by 3D-culture. PLoS One 9:e86372 (2014). ICC/IF ; Human . Read more (PubMed: 24466060) »
  • van Doremalen N  et al. Host species restriction of Middle East respiratory syndrome coronavirus through its receptor, dipeptidyl peptidase 4. J Virol 88:9220-32 (2014). Read more (PubMed: 24899185) »

See all 5 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Sample Mouse Tissue lysate - whole (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 02 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer pH 6.0
Sample Mouse Tissue sections (liver)
Specification liver
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 May 05 2014

En principio ab28340 detecta una banda a un peso algo superior al esperado debido a modificaciones post translacionales, el otro, ab114033 muestra una banda a un peso molecular menor que también aparece en la calle que contiene el control a much...

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees ...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Cow Tissue lysate - whole (bovine epididymis)
Loading amount 40 µg
Specification bovine epididymis
Treatment homogenized in 0.02 M Hepes buffer, pH 7.0, containing 1% octylglucoside and a complete protease inhibitor cocktail tablet
Gel Running Conditions Reduced Denaturing (7,5% gel)
Blocking step 0.05 M Tris buffer containing 0.15 M NaCl, 0.1% Tween 20, 5% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. Veronique Dubois

Verified customer

提交于 Aug 05 2009

Application Western blot
Sample Human Purified protein (human seminal plasma)
Loading amount 0.32 µg
Specification human seminal plasma
Gel Running Conditions Reduced Denaturing (7,5% gel)
Blocking step 0.05 M Tris buffer containing 0.15 M NaCl, 5 mM NaN3 and 2% Tween 20 as blocking agent for 1 hour(s) and 0 minute(s) · Temperature: 25°C
Username

Dr. Veronique Dubois

Verified customer

提交于 Jun 29 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Epididymis)
Specification Epididymis
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer pH 9.0
Permeabilization No
Blocking step 3% H2O2 in TBS as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Veronique Dubois

Verified customer

提交于 Jun 17 2009

Thank you for your enquiry. I have been in contact with the originator of these antibodies, and they are 99% homologous to chimp and so they should recognize your species. I hope this information helps, please do not hesitate to contact us if you n...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"