Anti-DPP4抗体[OTI11D7] (ab114033)
Key features and details
- Mouse monoclonal [OTI11D7] to DPP4
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG1
概述
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产品名称
Anti-DPP4抗体[OTI11D7]
参阅全部 DPP4 一抗 -
描述
小鼠单克隆抗体[OTI11D7] to DPP4 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human DPP4.
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阳性对照
- IHC-P: Liver tissue Flow Cyt: Hela cells WB: Transfected HEK293T cell lysate ICC/IF: Transfected COS7 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA -
Concentration information loading...
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纯度
IgG fraction -
纯化说明
Purified from TCS by affinity chromatography -
克隆
单克隆 -
克隆编号
OTI11D7 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab114033于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500. Predicted molecular weight: 88 kDa.
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IHC-P |
1/50.
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Flow Cyt |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/100.
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说明 |
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WB
1/500. Predicted molecular weight: 88 kDa. |
IHC-P
1/50. |
Flow Cyt
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/100. |
靶标
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功能
Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. -
组织特异性
Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon. -
序列相似性
Belongs to the peptidase S9B family. DPPIV subfamily. -
结构域
The extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity. -
翻译后修饰
The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
N- and O-Glycosylated.
Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation. -
细胞定位
Cell membrane. Apical cell membrane. Cell projection > invadopodium membrane. Cell projection > lamellipodium membrane. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface and Secreted. Detected in the serum and the seminal fluid. - Information by UniProt
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数据库链接
- Entrez Gene: 1803 Human
- Omim: 102720 Human
- SwissProt: P27487 Human
- Unigene: 368912 Human
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别名
- CD26 antigen antibody
- ADA-binding protein antibody
- ADABP antibody
see all
图片
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All lanes : Anti-DPP4 antibody [OTI11D7] (ab114033) at 1/500 dilution
Lane 1 : HEK293T cells were transfected with the pCMV6-ENTRY control cDNA for 48 hrs and lysed
Lane 2 : HEK293T cells were transfected with the pCMV6-ENTRY DPP4 cDNA for 48 hrs and lysed
Lysates/proteins at 5 µg per lane.
Predicted band size: 88 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC209466) -
Immunohistochemical staining of paraffin embedded liver tissue using ab114033 at 1/50
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Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DPP4, using ab114033 at 1/100
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Flow cytometric analysis of Hela cells, using ab114033 at 1/100 (Red) compared to a nonspecific negative control antibody (Blue)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab114033 被引用在 9 文献中.
- Shibasaki I et al. Serum and Adipose Dipeptidyl Peptidase 4 in Cardiovascular Surgery Patients: Influence of Dipeptidyl Peptidase 4 Inhibitors. J Clin Med 11:N/A (2022). PubMed: 35893426
- Chen W et al. Roles of the SNHG7/microRNA-9-5p/DPP4 ceRNA network in the growth and 131I resistance of thyroid carcinoma cells through PI3K/Akt activation. Oncol Rep 45:N/A (2021). PubMed: 33649840
- Fahrner M et al. Proteome biology of primary colorectal carcinoma and corresponding liver metastases. Neoplasia 23:1240-1251 (2021). PubMed: 34768110
- Fang L et al. OM-85 Broncho-Vaxom®, a Bacterial Lysate, Reduces SARS-CoV-2 Binding Proteins on Human Bronchial Epithelial Cells. Biomedicines 9:N/A (2021). PubMed: 34829773
- Zhou LJ et al. Toxoplasma gondii SAG1 targeting host cell S100A6 for parasite invasion and host immunity. iScience 24:103514 (2021). PubMed: 34950858
- Song Y et al. Expression of GLP-1 receptor and CD26 in human thyroid C-cells: The association of thyroid C-cell tumorigenesis with incretin-based medicine. Oncol Lett 13:2684-2690 (2017). IHC-P ; Human . PubMed: 28454451
- Lee JJ et al. Dipeptidyl Peptidase IV as a Prognostic Marker and Therapeutic Target in Papillary Thyroid Carcinoma. J Clin Endocrinol Metab 102:2930-2940 (2017). PubMed: 28575350
- Zheng B et al. A three-gene panel that distinguishes benign from malignant thyroid nodules. Int J Cancer 136:1646-54 (2015). PubMed: 25175491
- Rainczuk A et al. Evidence for the antagonistic form of CXC-motif chemokine CXCL10 in serous epithelial ovarian tumours. Int J Cancer 134:530-41 (2014). PubMed: 23873303