The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.25-0.5µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended.
Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
Genetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Note=The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T cell-APC interaction and the functional consequences of T cell activation, thereby destabilizing peripheral tolerance.
Flow cytometry staining of 3-day unstimulated (left) and 3-day Anti-Mouse IgM and Anti-Mouse CD40 stimulated (right) BALB/c splenocytes with 0.25 µg of Rat IgG2b ? Isotype Control PE (open histogram) or 0.25 µg of ab95656 (filled histogram). Total viable cells were used for analysis.
Tsukada N et al. Blockade of CD134 (OX40)-CD134L interaction ameliorates lethal acute graft-versus-host disease in a murine model of allogeneic bone marrow transplantation. Blood95:2434-9 (2000).
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Akiba H et al. Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis. J Exp Med191:375-80 (2000).
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