Anti-CD252抗体[MM0505-8S23] (ab89896)

概述

  • 产品名称Anti-CD252抗体[MM0505-8S23]
    参阅全部 CD252 一抗
  • 描述
    小鼠单克隆抗体[MM0505-8S23] to CD252
  • 经测试应用适用于: IHC-P, IHC-Fr, Neutralising, Flow Cytmore details
  • 种属反应性
    与反应: Human, Cynomolgus Monkey
  • 免疫原

    Recombinant full length Human CD252

  • 常规说明The antibody is specific to OX40L.

性能

应用

Our Abpromise guarantee covers the use of ab89896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/20 - 1/200.
Neutralising Use at an assay dependent concentration. Proliferation of human PBMCs elicited by TNFSF4 (25ng/mL) can be neutralized by increasing concentrations of ab89896 in a dose-dependent manner. The ED50 is between 40-80ng/mL.
Flow Cyt 1/50 - 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
  • 疾病相关Genetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Note=The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T cell-APC interaction and the functional consequences of T cell activation, thereby destabilizing peripheral tolerance.
  • 序列相似性Belongs to the tumor necrosis factor family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 134L antibody
    • CD 252 antibody
    • CD134 antibody
    • CD134 ligand antibody
    • CD134L antibody
    • CD252 antibody
    • CD252 antigen antibody
    • glycoprotein 34 kd antibody
    • Glycoprotein Gp34 antibody
    • GP34 antibody
    • OX-40L antibody
    • OX40 antigen ligand antibody
    • OX40 ligand antibody
    • OX40L antibody
    • TAX transcriptionally-activated glycoprotein 1 antibody
    • TNFL4_HUMAN antibody
    • Tnfsf4 antibody
    • Tumor necrosis factor (ligand) superfamily member 4 antibody
    • Tumor necrosis factor ligand superfamily member 4 antibody
    • TXGP1 antibody
    see all

Anti-CD252 antibody [MM0505-8S23] 图像

  • ab89896 staining CD252 in Cynomolgus Monkey lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 20 minutes at room temperature; antigen retrieval was enzymatic. Samples were incubated with primary antibody (1/25) for 30 minutes at room temperture. An undiluted Biotin-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

Anti-CD252 antibody [MM0505-8S23] (ab89896)参考文献

ab89896 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: Rt°C
Antigen retrieval step Enzymatic
Sample Cynomolgus Monkey Tissue sections (Lymph node)
Specification Lymph node
Permeabilization No
Fixative Formaldehyde
Username

Jing Ma

Verified customer

提交于 Aug 30 2013

Thank you very much for your interest in ab89896.

This clone was tested using frozen human inflammatory lymph nodes by immunofluorescence, showing positive subgroup cells.


We do not currently have an image of ab89896 in IHC-Fr...

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For each lot of ab89896, the level of LPS is <0.01EU/ug IgG.

Thank you for your email.

Here are some answers:

1. Did Abcam use fresh or thawed PBMC?

We used freshed ones!

2. How many PBMC were culture in each well?, what media did Abcam use? Did they
used activation apart ...

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Thank you for contacting us.

The endotoxin level is <0.5EU/mg antibody.

The antibody is azide free.

Customer needs optimizing the conditions on his/her own as the culture condition wont be the same. The assay was performed...

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Thank you for patience while I contacted the lab.
Unfortunately we do not have a detailed protocol for this antibody
In essence they use the primary antibody at2.5ug per1 x 10(6) cells (human PBMCs stimulated with cytokines) and then follow a...

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Thank you for your interest in ab89896. I have confirmed with the laboratory that the endotoxin level is <0.5EU/mg antibody. I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

Human recombinant TNFSF4 (with Poly-His taq) can stimulate the proliferation of human PBMCs, in the presence of cross-linking antibody and PHA (phytohemagglutinin). Under this condition, proliferation of human PBMCs elicited by TNFSF4 (25ng/mL) can...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"