Anti-CD24抗体[SN3] (Phycoerythrin) (ab77219)

概述

  • 产品名称Anti-CD24抗体[SN3] (Phycoerythrin)
    参阅全部 CD24 一抗
  • 描述
    小鼠单克隆抗体[SN3] to CD24 (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Glycoproteins purified from human NALM-1 cell line.

  • 阳性对照
    • Human blood cells.
  • 常规说明

    Purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

应用

Our Abpromise guarantee covers the use of ab77219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明Flow Cyt: Use 20µl per 100µl of whole blood or 106 cells in suspension.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.
    • 组织特异性B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.
    • 疾病相关Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface.
    • 序列相似性Belongs to the CD24 family.
    • 翻译后修饰Extensively O-glycosylated.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD 24 antibody
      • CD24 antibody
      • CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody
      • CD24 antigen antibody
      • CD24 molecule antibody
      • CD24_HUMAN antibody
      • CD24A antibody
      • FLJ22950 antibody
      • FLJ43543 antibody
      • GPI linked surface mucin antibody
      • Heat stable antigen antibody
      • HSA antibody
      • MGC75043 antibody
      • Nectadrin antibody
      • Signal transducer CD24 antibody
      • Small cell lung carcinoma cluster 4 antigen antibody
      see all

    Anti-CD24 antibody [SN3] (Phycoerythrin) 图像

    • ab77219 staining human MCF7 cells by Flow Cytometry. The primary antibody was diluted 1/5 and incubated with sample for 30 minutes at 4°C. The purple profile shows negative control (mouse IgG) vs. green profile ab77219 (CD24).

      See Abreview

    Anti-CD24 antibody [SN3] (Phycoerythrin) (ab77219)参考文献

    This product has been referenced in:
    • Moschidou D  et al. Human mid-trimester amniotic fluid stem cells cultured under embryonic stem cell conditions with Valproic acid acquire pluripotent characteristics. Stem Cells Dev : (2012). Flow Cyt ; Human . Read more (PubMed: 23050522) »
    • Maliar A  et al. Redirected T cells that target pancreatic adenocarcinoma antigens eliminate tumors and metastases in mice. Gastroenterology 143:1375-1384.e5 (2012). Read more (PubMed: 22819865) »

    See all 6 Publications for this product

    Product Wall

    Thank you for your enquiry regardingab77219 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

    CD24 is a cell surface marker and it is a high cha...

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    Thank you for contacting us.

    The antibody concentration is 30 ug/ml. Thus, 1 vial contains 30 ug.

    For any bulk order, please contact our bulk specialist at sales@abcam.com

    I hope this information is helpful to you. Pleas...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (MCF-7)
    Specification MCF-7
    Preparation Cell harvesting/tissue preparation method: Cell dissociation buffer
    Sample buffer: PBS + 0.2% BSA + 0.1% Sodium azide
    Fixation Paraformaldehyde
    Permeabilization No
    Gating Strategy Live cells
    Username

    Abcam user community

    Verified customer

    提交于 May 24 2012

    Thank you for contacting Abcam

    The antibody ab77219 is supplied at 18ug/ml and although the lab did not provide the total volume supplied, we do guarantee that there will be enough volume present to carry out 100 tests and if you happen to be...

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    Code de remise : 123456789 Date d’expiration : JJ MM AAAA Nous sommes ravis que vous souhaitiez participer à notre offre et tester ab77219 chez le singe. Le code de réduction correspondant est 123456789. Ce code de réduction est utilisable s...

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    Thanks for your reply and for the information. Ab77219 and ab81424 have been tested in flow cytometry and are guaranteed to work in FC with human cells. You are correct, they have not been tested in ICC/IF, and it is possible that they will wor...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"