The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 0.25µg for 106 cells in 100µl volume.
FuncS: Use at an assay dependent dilution. in vitro induction of thymocyte maturation2 and complement-mediated cytotoxicity.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution. Use zinc-fixed paraffin-embedded sections or formalin-fixed paraffin-embedded sections.
IHC-Fr: Use at an assay dependent dilution. Use acetone-fixed frozen sections.
WB: Use at an assay dependent dilution. Predicted molecular weight: 9 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.
B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.
Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface.
Flow cytometry of C57BL/6 mouse splenocytes stained with ab64064, at 0.25 µg per 106 cells, followed by anti-rat IgG FITC.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD24 antibody [M1/69] (ab64064)This image is courtesy of an Abreview submitted by Mr Rudolf Jung
Immunohistochemical analysis of Mouse spleen tissue, staining Perforin with ab64064.
Tissue was fixed with paraformaldehyde and permeabilized with Tween-20; antigen retrieval was by heat mediation in Tris-EDTA buffer. Samples were incubated with primary antibody (1/50 in diluent) for 30 minutes at 20°C. An undiluted Histofine®-conjugated goat anti-rat polyclonal IgG was used as the secondary antibody.