概述

  • 产品名称Anti-CD24抗体[M1/69]
    参阅全部 CD24 一抗
  • 描述
    大鼠单克隆抗体[M1/69] to CD24
  • 经测试应用适用于: WB, IHC-Fr, Flow Cyt, IHC-P, Functional Studies, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes.

  • 阳性对照
    • C57BL/6 mouse splenocytes.

应用

Our Abpromise guarantee covers the use of ab64064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-Fr
Flow Cyt
IHC-P
Functional Studies
ICC/IF
  • 应用说明Flow Cyt: Use 0.25µg for 106 cells in 100µl volume.
    FuncS: Use at an assay dependent dilution. in vitro induction of thymocyte maturation2 and complement-mediated cytotoxicity.
    IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution. Use zinc-fixed paraffin-embedded sections or formalin-fixed paraffin-embedded sections.
    IHC-Fr: Use at an assay dependent dilution. Use acetone-fixed frozen sections.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 9 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.
    • 组织特异性B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.
    • 疾病相关Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface.
    • 序列相似性Belongs to the CD24 family.
    • 翻译后修饰Extensively O-glycosylated.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD 24 antibody
      • CD24 antibody
      • CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody
      • CD24 antigen antibody
      • CD24 molecule antibody
      • CD24_HUMAN antibody
      • CD24A antibody
      • FLJ22950 antibody
      • FLJ43543 antibody
      • GPI linked surface mucin antibody
      • Heat stable antigen antibody
      • HSA antibody
      • MGC75043 antibody
      • Nectadrin antibody
      • Signal transducer CD24 antibody
      • Small cell lung carcinoma cluster 4 antigen antibody
      see all

    Anti-CD24 antibody [M1/69] 图像

    • Flow cytometry of C57BL/6 mouse splenocytes stained with ab64064, at 0.25 µg per 106 cells, followed by anti-rat IgG FITC.
    • Immunohistochemical analysis of Mouse spleen tissue, staining Perforin with ab64064.

      Tissue was fixed with paraformaldehyde and permeabilized with Tween-20; antigen retrieval was by heat mediation in Tris-EDTA buffer. Samples were incubated with primary antibody (1/50 in diluent) for 30 minutes at 20°C. An undiluted Histofine®-conjugated goat anti-rat polyclonal IgG was used as the secondary antibody.

      See Abreview

    Anti-CD24 antibody [M1/69] (ab64064)参考文献

    This product has been referenced in:
    • Cuevas E  et al. Lin41/Trim71 is essential for mouse development and specifically expressed in postnatal ependymal cells of the brain. Front Cell Dev Biol 3:20 (2015). Read more (PubMed: 25883935) »
    • Neirinckx V  et al. Adult Bone Marrow Neural Crest Stem Cells and Mesenchymal Stem Cells Are Not Able to Replace Lost Neurons in Acute MPTP-Lesioned Mice. PLoS One 8:e64723 (2013). IHC-Fr, ICC/IF ; Mouse . Read more (PubMed: 23741377) »

    See all 2 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Kidney Progemitor Cells)
    Permeabilization No
    Specification Kidney Progemitor Cells
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Ms. Maria Pearce

    Verified customer

    提交于 Aug 01 2016

    Application Immunoprecipitation
    Immuno-precipitation step Protein A/G
    Sample Human Cell lysate - whole cell (MDA-MB-231)
    Specification MDA-MB-231
    Total protein in input 30 µg
    Username

    Abcam user community

    Verified customer

    提交于 May 01 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
    Sample Human Tissue sections (human colon)
    Specification human colon
    Permeabilization Yes - tween-20
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 May 01 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample Human Cell (MCF7)
    Specification MCF7
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 May 01 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Spleen)
    Specification Spleen
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA-Buffer pH 9,0
    Permeabilization Yes - Wash-Buffer from Dako with Tween
    Username

    Mr. Rudolf Jung

    Verified customer

    提交于 Oct 01 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"