概述

  • 产品名称Anti-CD24抗体[ALB 9]
    参阅全部 CD24 一抗
  • 描述
    小鼠单克隆抗体[ALB 9] to CD24
  • 特异性The clone number has been updated from (8.B.76) to (ALB 9) both clone numbers name the same antibody clone.
  • 经测试应用适用于: ICC, Flow Cyt, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human bone marrow malignant cells from a non-B, non-T acute leukemia. MW: 35-45kD.

  • 阳性对照
    • Flow cytometry: Live HAP1 wildtype and HAP1-CD24 KO cells.
  • 常规说明

    CD24 expression can also be observed in the cytoplasm.

    ab31622 binds to the non-glycosylated GPI anchor of the protein core of the cluster w4/CD24. It recognizes B cells from pre-B stage to mature-B stage, the majority of normal medullar granulous cells, peripheral blood polymorphonuclear cells, non-T, non-B acute lymphoblastic leukemias, some atypic or myeloid leukemias or B chronic lymphocytic leukemias and two-thirds of Epstein-Barr virus transformed cell lines.

性能

应用

Our Abpromise guarantee covers the use of ab31622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/25 - 1/50. Cell smear.
Flow Cyt Use a concentration of 10 µg/ml.

ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/25 - 1/50.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.
  • 组织特异性B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.
  • 疾病相关Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface.
  • 序列相似性Belongs to the CD24 family.
  • 翻译后修饰Extensively O-glycosylated.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 24 antibody
    • CD24 antibody
    • CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody
    • CD24 antigen antibody
    • CD24 molecule antibody
    • CD24_HUMAN antibody
    • CD24A antibody
    • FLJ22950 antibody
    • FLJ43543 antibody
    • GPI linked surface mucin antibody
    • Heat stable antigen antibody
    • HSA antibody
    • MGC75043 antibody
    • Nectadrin antibody
    • Signal transducer CD24 antibody
    • Small cell lung carcinoma cluster 4 antigen antibody
    see all

Anti-CD24 antibody [ALB 9] 图像

  • Overlay histogram showing HAP1 wildtype (green line) and HAP1-CD24 knockout cells (red line) stained with ab31622. Live HAP1 wildtype and HAP1-CD24 knockout cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab31622, 10µg/0.5x106 cells) for 30 min at 22°C. A mouse IgG1 isotype control antibody  (ab170190) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-ITGB1 knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity). Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

  • ab31622 staining CD24 in human colon tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde, then a heat mediated antigen retrieval step was performed using citrate buffer pH 6 (20 minutes). Samples were then incubated with the primary antibody at a 1/100 dilution for 16 minutes at 25°C. An undiluted HRP-conjugated goat anti-mouse/rabbit monoclonal was used as secondary antibody.

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Anti-CD24 antibody [ALB 9] (ab31622)参考文献

This product has been referenced in:
  • Chen D  et al. Role of medullary progenitor cells in epithelial cell migration and proliferation. Am J Physiol Renal Physiol 307:F64-74 (2014). Read more (PubMed: 24808539) »
  • Rasmussen JG  et al. Activation of Protease-Activated Receptor 2 Induces VEGF Independently of HIF-1. PLoS One 7:e46087 (2012). Flow Cyt ; Human . Read more (PubMed: 23049945) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Ph=6
Sample Human Tissue sections (Normal breast tissue, MCF-7 and MDA-MB-231 cell li)
Specification Normal breast tissue, MCF-7 and MDA-MB-231 cell li
Permeabilization No
Fixative Formaldehyde
Username

Dr. Hazem Ghebeh

Verified customer

提交于 Sep 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (colon, normal and cancer)
Specification colon, normal and cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH6, 20 min
Permeabilization No
Username

Abcam user community

Verified customer

提交于 May 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"