Anti-CD22抗体[RFB4] (Phycoerythrin) (ab23620)


  • 产品名称Anti-CD22抗体[RFB4] (Phycoerythrin)
    参阅全部 CD22 一抗
  • 描述
    小鼠单克隆抗体[RFB4] to CD22 (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特异性CD22 is in principle a B lineage antigen, that is present in the cytoplasm of progenitor B-cells and on the membrane of the majority of mature peripheral B-lymphocytes. Furthermore, it is strongly expressed on Hairy Cell Leukemia cells and very weakly on some other leukemias. Antigen distribution: Peripheral blood lymphocytes 12±3% T-cells (E+) < 1% B-cells (E-, Ig+) 92±3% Monocytes (CD14+) < 1% Granulocytes < 1% Thymocytes < 1%
  • 经测试应用适用于: IHC-Fr, Flow Cytmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified tonsil lymphocytes (Human).



Our Abpromise guarantee covers the use of ab23620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 5µl for 106 cells.

Use maximum of 5µl for 106 cells.

ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
    • 组织特异性B-lymphocytes.
    • 序列相似性Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • 结构域Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • 翻译后修饰Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
      Phosphorylated on tyrosine residues by LYN.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • B cell receptor CD22 precursor antibody
      • B lymphocyte cell adhesion molecule antibody
      • B-cell receptor CD22 antibody
      • B-lymphocyte cell adhesion molecule antibody
      • BL CAM antibody
      • BL-CAM antibody
      • BLCAM antibody
      • CD 22 antibody
      • CD22 antibody
      • CD22 antigen antibody
      • CD22 molecule antibody
      • CD22 protein antibody
      • CD22_HUMAN antibody
      • Lectin 2 antibody
      • Leu14 antibody
      • Lyb8 antibody
      • MGC130020 antibody
      • sialic acid binding Ig like lectin 2 antibody
      • Sialic acid binding immunoglobulin like lectin 2 antibody
      • Sialic acid-binding Ig-like lectin 2 antibody
      • SIGLEC 2 antibody
      • Siglec-2 antibody
      • SIGLEC2 antibody
      • T cell surface antigen Leu 14 antibody
      • T-cell surface antigen Leu-14 antibody
      see all

    Anti-CD22 antibody [RFB4] (Phycoerythrin) (ab23620)参考文献

    This product has been referenced in:
    • Ito D  et al. Exclusion of cytoplasmic fragments in flow cytometric analysis of lymph node samples from dogs with lymphoma using membrane-permeable violet laser-excitable DNA-binding fluorescent dye (DyeCycle Violet). Vet Clin Pathol 39:494-8 (2010). Read more (PubMed: 21198735) »

    See 1 Publication for this product

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