Anti-CD22抗体[MEM-01] (ab2526)

概述

  • 产品名称Anti-CD22抗体[MEM-01]
    参阅全部 CD22 一抗
  • 描述
    小鼠单克隆抗体[MEM-01] to CD22
  • 特异性The antibody MEM-01 stains only the zone corresponding to 140 kDa, but not the weaker 130 kDa zone (stained by several CD22 antibodies recognizing both isoforms).
  • 经测试应用适用于: Flow Cyt, IP, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Non Human Primates
  • 免疫原

    Tissue/ cell preparation (Human). Burkitt's lymphoma cell line Raji.

  • 表位Although not specifically mapped, this antibody blocks anti-CD22 monoclonal antibody OTH228, which uniquely recognizes epitope "E". It does not, however, block anti-CD22 monoclonal antibodies RFB 4, CLB22/1 and CLB-BLy1.
  • 阳性对照
    • Raji human lymphoma cell line.

性能

应用

Our Abpromise guarantee covers the use of ab2526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use a concentration of 2 - 4 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 140 kDa. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.

靶标

  • 功能Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
  • 组织特异性B-lymphocytes.
  • 序列相似性Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 结构域Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • 翻译后修饰Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
    Phosphorylated on tyrosine residues by LYN.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B cell receptor CD22 precursor antibody
    • B lymphocyte cell adhesion molecule antibody
    • B-cell receptor CD22 antibody
    • B-lymphocyte cell adhesion molecule antibody
    • BL CAM antibody
    • BL-CAM antibody
    • BLCAM antibody
    • CD 22 antibody
    • CD22 antibody
    • CD22 antigen antibody
    • CD22 molecule antibody
    • CD22 protein antibody
    • CD22_HUMAN antibody
    • Lectin 2 antibody
    • Leu14 antibody
    • Lyb8 antibody
    • MGC130020 antibody
    • sialic acid binding Ig like lectin 2 antibody
    • Sialic acid binding immunoglobulin like lectin 2 antibody
    • Sialic acid-binding Ig-like lectin 2 antibody
    • SIGLEC 2 antibody
    • Siglec-2 antibody
    • SIGLEC2 antibody
    • T cell surface antigen Leu 14 antibody
    • T-cell surface antigen Leu-14 antibody
    see all

Anti-CD22 antibody [MEM-01] 图像

  • Flow cytometry analysis of human peripheral blood leukocytes with anti-human CD22 (MEM-01) purified. 

Anti-CD22 antibody [MEM-01] (ab2526)参考文献

ab2526 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab2526.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"