重组
RabMAb

Anti-CD21抗体[EP3093] (ab75985)

概述

  • 产品名称
    Anti-CD21抗体[EP3093]
    参阅全部 CD21 一抗
  • 描述
    兔单克隆抗体[EP3093] to CD21
  • 经测试应用
    适用于: ICC/IF, WB, IP, IHC-P, Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide within Human CD21 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.

  • 阳性对照
    • Daudi cell lysate, Raji cell lysate and Tonsil tissue
  • 常规说明

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    EP3093
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/250 - 1/500.
WB 1/2000 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 113 kDa).
IP 1/50.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能
    Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation.
  • 组织特异性
    Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.
  • 疾病相关
    Genetic variations in CR2 are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9) [MIM:610927]. Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • 序列相似性
    Belongs to the receptors of complement activation (RCA) family.
    Contains 15 Sushi (CCP/SCR) domains.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C3DR antibody
    • CD 21 antibody
    • CD21 antibody
    • Complement C3d receptor 2 antibody
    • Complement C3d receptor antibody
    • Complement component (3d/Epstein Barr virus) receptor 2 antibody
    • Complement receptor type 2 antibody
    • CR antibody
    • Cr2 antibody
    • CR2_HUMAN antibody
    • CVID7 antibody
    • EBV receptor antibody
    • EBV-R antibody
    • Epstein Barr virus receptor antibody
    • Epstein-Barr virus receptor antibody
    • EVBR antibody
    • SLEB9 antibody
    see all

Anti-CD21 antibody [EP3093] 图像

  • ab75985 showing positive staining in Normal tonsil tissue.

  • ab75985 staining CD21 antibody in Raji (human Burkitt's lymphoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

    DAPI was used as a nuclear counterstain and the negative control was PBS only.

  • All lanes : Anti-CD21 antibody [EP3093] (ab75985) at 1/2000 dilution

    Lane 1 : Daudi cell lysate
    Lane 2 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 113 kDa
    Observed band size : 155 kDa (why is the actual band size different from the predicted?)
  • ab75985 staining CD21 in Cynomolgus Monkey whole blood by Flow Cytometry. Red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide, fixed in paraformaldehyde and incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
    Gating Strategy: Live Lymphocytes.

    See Abreview

  • ab75985 showing negative staining in Normal liver tissue.

  • ab75985 showing negative staining in Normal kidney tissue.

  • ab75985 showing negative staining in Normal brain tissue.

  • ab75985 showing negative staining in Normal breast tissue.

Anti-CD21 antibody [EP3093] (ab75985)参考文献

This product has been referenced in:
  • Furukawa S  et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017). IHC ; Monkey . Read more (PubMed: 28190929) »
  • Sato Y  et al. Heterogeneous fibroblasts underlie age-dependent tertiary lymphoid tissues in the kidney. JCI Insight 1:e87680 (2016). IF ; Mouse, Human . Read more (PubMed: 27699223) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (PBMC)
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: form buffy coat after FICOLL
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
lymphocyte gated
Username

Abcam user community

Verified customer

提交于 Nov 01 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Rat Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: spleen was mashed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

提交于 Apr 19 2012

Thank you for submitting an Abreview of ab75985. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this...

Read More
Application
Flow Cytometry
Sample
Mouse Cell (splenocytes)
Specification
splenocytes
Preparation
Cell harvesting/tissue preparation method: after spleen mashing
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

提交于 Apr 18 2012

Application
Flow Cytometry
Sample
Cynomolgus Monkey Cell (Whole blood)
Specification
Whole blood
Preparation
Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

提交于 Apr 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (spleen from immune mice)
Specification
spleen from immune mice
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 13 2012

Yes, the epitope of this antibody is intracellular. Therefore it will be necessary to permeabilize the cells for flow cytometry.

Thank you for contacting Abcam. I can provide the broader range of the immunogen for the blocking peptide. The peptide is derived from the terminal region, amino acid ***********of human CD21 (UniProt: P20023). I am sorry that I am unable to provide a ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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