概述

  • 产品名称Anti-CD21抗体[Bu32]
    参阅全部 CD21 一抗
  • 描述
    小鼠单克隆抗体[Bu32] to CD21
  • 特异性ab54253 recognises human CD21.
  • 经测试应用适用于: IP, Flow Cyt, ICC/IF, IHC-Frmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation (Human)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号Bu32
  • 骨髓瘤NS0
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab54253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells.

1/50 - 1/200. Use 10µl of the suggested working dilution to label 1x10^6 cells in 100µl.

 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation.
    • 组织特异性Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.
    • 疾病相关Genetic variations in CR2 are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9) [MIM:610927]. Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
    • 序列相似性Belongs to the receptors of complement activation (RCA) family.
      Contains 15 Sushi (CCP/SCR) domains.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C3DR antibody
      • CD 21 antibody
      • CD21 antibody
      • Complement C3d receptor antibody
      • Complement component (3d/Epstein Barr virus) receptor 2 antibody
      • Complement receptor type 2 antibody
      • CR antibody
      • Cr2 antibody
      • CR2_HUMAN antibody
      • CVID7 antibody
      • EBV receptor antibody
      • EBV-R antibody
      • Epstein Barr virus receptor antibody
      • Epstein-Barr virus receptor antibody
      • EVBR antibody
      • SLEB9 antibody
      see all

    Anti-CD21 antibody [Bu32] 图像

    • Human peripheral blood lymphocytes stained with ab54253 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab54253, 0.1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

    Anti-CD21 antibody [Bu32] (ab54253)参考文献

    This product has been referenced in:
    • Herishanu Y  et al. Divergence in CD19-mediated signaling unfolds intraclonal diversity in chronic lymphocytic leukemia, which correlates with disease progression. J Immunol 190:784-93 (2013). Read more (PubMed: 23241880) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"