The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1 - 1/10.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Receptor for the CD200/OX2 cell surface glycoprotein. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.
Expressed in granulocytes, monocytes, most T cells, neutrophils, basophils and a subset of NK, NKT and B cells (at protein level). Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells.
Belongs to the CD200R family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
The mature form of isoform 2 and/or isoform 4 starts at sequence position 27 of the corresponding isoform.
Flow Cytometry - CD200R antibody [OX102] (Phycoerythrin) (ab34135)This image is courtesy of an Anonymous Abreview.
Overlay histogram showing Rat blood cells stained with ab34135 (red line). The sample was prepared by lysing Red Blood cells and cells were fixed with formaldehyde. The cells were then incubated with the antibody for 1 hour diluted 1/100 in PBS/1% BSA/0.01% Azide. The cell population was gated on monocytes.