Anti-CD200 / OX2抗体[OX-104] (ab23552)
Key features and details
- Mouse monoclonal [OX-104] to CD200 / OX2
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-CD200 / OX2抗体[OX-104]
参阅全部 CD200 / OX2 一抗 -
描述
小鼠单克隆抗体[OX-104] to CD200 / OX2 -
宿主
Mouse -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Fusion protein corresponding to Human CD200/ OX2 (extracellular). Recombinant fusion protein consisting of the extracellular region of human CD200/ OX2 fused with domains three and four of rat CD4. Full details of the immunogen can be found in PubMed ID 11260322.
Database link: P41217 -
阳性对照
- Flow Cyt: Human peripheral blood mononuclear cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
OX-104 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23552于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. -
序列相似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4345 Human
- Omim: 155970 Human
- SwissProt: P41217 Human
- Unigene: 79015 Human
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别名
- Antigen identified by monoclonal antibody MRC OX 2 antibody
- CD200 antibody
- CD200 antigen antibody
see all
图片
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Human peripheral blood mononuclear cells stained with ab23552 (right) or mouse IgG1 kappa (ab170190) isotype (left). Human peripheral blood mononuclear cells were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab23552) or mouse IgG1 kappa (ab170190) (1x106 in 100 µl at 1 µg/ml) for 30 min on ice. The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150177) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD19 APC antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab23552 被引用在 3 文献中.
- Zhou H et al. Feasibility of repairing full-thickness skin defects by iPSC-derived epithelial stem cells seeded on a human acellular amniotic membrane. Stem Cell Res Ther 10:155 (2019). PubMed: 31151466
- Adhikary G et al. Identification of a population of epidermal squamous cell carcinoma cells with enhanced potential for tumor formation. PLoS One 8:e84324 (2013). Human . PubMed: 24376802
- Rasmussen JG et al. Activation of Protease-Activated Receptor 2 Induces VEGF Independently of HIF-1. PLoS One 7:e46087 (2012). Flow Cyt ; Human . PubMed: 23049945