概述

  • 产品名称Anti-CD20抗体[EP459Y]
    参阅全部 CD20 一抗
  • 描述
    兔单克隆抗体[EP459Y] to CD20
  • 经测试应用适用于: WB, IP, IHC-Pmore details
    不适用于: Flow Cyt or ICC/IF
  • 种属反应性
    与反应: Human, Monkey
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD20 aa 250 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 阳性对照
    • Raji cell lysate. Human lymph node tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

应用

Our Abpromise guarantee covers the use of ab78237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IP 1/20.
IHC-P 1/50 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or ICC/IF.
  • 靶标

    • 功能This protein may be involved in the regulation of B-cell activation and proliferation.
    • 组织特异性Expressed on B-cells.
    • 疾病相关Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
    • 序列相似性Belongs to the MS4A family.
    • 翻译后修饰Phosphorylated. Might be functionally regulated by protein kinase(s).
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • APY antibody
      • ATOPY antibody
      • B lymphocyte antigen CD20 antibody
      • B Lymphocyte Cell Surface Antigen B1 antibody
      • B-lymphocyte antigen CD20 antibody
      • B-lymphocyte cell-surface antigen B1 antibody
      • B-lymphocyte surface antigen B1 antibody
      • B1 antibody
      • Bp 35 antibody
      • Bp35 antibody
      • CD 20 antibody
      • CD20 antibody
      • CD20 antigen antibody
      • CD20 receptor antibody
      • CD20_HUMAN antibody
      • CVID5 antibody
      • Fc epsilon receptor I beta chain antibody
      • Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody
      • FCER1B antibody
      • High affinity immunoglobulin epsilon receptor subunit beta antibody
      • IgE Fc receptor subunit beta antibody
      • IGEL antibody
      • IGER antibody
      • IGHER antibody
      • LEU 16 antibody
      • LEU16 antibody
      • leukocyte surface antigen Leu 16 antibody
      • Leukocyte surface antigen Leu-16 antibody
      • Ly44 antibody
      • Membrane spanning 4 domains A1 antibody
      • Membrane spanning 4 domains subfamily A member 2 antibody
      • Membrane-spanning 4-domains subfamily A member 1 antibody
      • MS4A1 antibody
      • MS4A2 antibody
      • S7 antibody
      see all

    Anti-CD20 antibody [EP459Y] 图像

    • Unpurified ab78237 showing positive staining in normal tonsil tissue.

    • Immunohistochemical staining of paraffin embedded B cell lymphoma with purified ab78237 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Anti-CD20 antibody [EP459Y] (ab78237) at 1/10000 dilution (purified) + Raji whole cell lysate

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 33 kDa
      Observed band size : 33 kDa

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Unpurified ab78237 staining human CD20 in human lymphoma tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.

    • ab78237 (purified) at 1/20 immunoprecipitating CD20 in 10 μg Ramos cell lysate (Lanes 1 and 2, observed at 33 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
    • Anti-CD20 antibody [EP459Y] (ab78237) at 1/5000 dilution (unpurified) + Raji cell lysate

      Predicted band size : 33 kDa
      Observed band size : 33 kDa
    • Unpurified ab78237 showing positive staining in normal spleen tissue.

    • Unpurified ab78237 showing negative staining in normal brain tissue.

    • Unpurified ab78237 showing negative staining in normal heart tissue.

    • Unpurified ab78237 showing negative staining in normal kidney tissue.

    Anti-CD20 antibody [EP459Y] (ab78237)参考文献

    This product has been referenced in:
    • Shi H  et al. Long non-coding RNA expression profile in minor salivary gland of primary Sjögren's syndrome. Arthritis Res Ther 18:109 (2016). IHC ; Human . Read more (PubMed: 27188286) »
    • Toyoshima K  et al. Analysis of circulating tumor cells derived from advanced gastric cancer. Int J Cancer 137:991-8 (2015). IHC-P ; Human . Read more (PubMed: 25622566) »

    See all 9 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Monkey Tissue sections (Marmoset spleen)
    Specification Marmoset spleen
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Username

    Mr. Carl Hobbs

    Verified customer

    提交于 Jul 19 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Spleen)
    Specification Spleen
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid ph6
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
    Username

    Mr. Carl Hobbs

    Verified customer

    提交于 Jul 19 2010

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Spleen)
    Permeabilization Yes - Tween
    Specification Spleen
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Fixative Formaldehyde
    Username

    Dr. Andrea Vecchione

    Verified customer

    提交于 Sep 07 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BlockAid as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric buffer, pH 6
    Sample Human Tissue sections (Skin)
    Specification Skin
    Permeabilization Yes - 0.2% Triton X-100
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Apr 06 2015

    Application Immunohistochemistry (Frozen sections)
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 22°C
    Sample Rhesus monkey Tissue sections (Lymph node)
    Specification Lymph node
    Permeabilization Yes - 0.5% Triton X
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jan 19 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Spleen)
    Specification Spleen
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Username

    Abcam user community

    Verified customer

    提交于 Apr 25 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"