Anti-CD20抗体[B9E9] (FITC) (ab1169)

概述

  • 产品名称Anti-CD20抗体[B9E9] (FITC)
    参阅全部 CD20 一抗
  • 描述
    小鼠单克隆抗体[B9E9] to CD20 (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 特异性It is strictly specific to an extracellular epitope of human CD20 antigen, present on normal peripheral B lymphocytes, malignant cells of pre-B acute lymphoblastic leukaemia patients and malignant cells of B-type chronic lymphoblastic leukaemia patients. It is not necessary to permeabilize the cells.
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Cells of lymphoblastoid cell line DAUDI

性能

应用

Our Abpromise guarantee covers the use of ab1169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1-5µl for 106 cells. Optimal volumes should be determined by the end user.

靶标

  • 功能This protein may be involved in the regulation of B-cell activation and proliferation.
  • 组织特异性Expressed on B-cells.
  • 疾病相关Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • 序列相似性Belongs to the MS4A family.
  • 翻译后修饰Phosphorylated. Might be functionally regulated by protein kinase(s).
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APY antibody
    • ATOPY antibody
    • B lymphocyte antigen CD20 antibody
    • B Lymphocyte Cell Surface Antigen B1 antibody
    • B-lymphocyte antigen CD20 antibody
    • B-lymphocyte cell-surface antigen B1 antibody
    • B-lymphocyte surface antigen B1 antibody
    • B1 antibody
    • Bp 35 antibody
    • Bp35 antibody
    • CD 20 antibody
    • CD20 antibody
    • CD20 antigen antibody
    • CD20 receptor antibody
    • CD20_HUMAN antibody
    • CVID5 antibody
    • Fc epsilon receptor I beta chain antibody
    • Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody
    • FCER1B antibody
    • High affinity immunoglobulin epsilon receptor subunit beta antibody
    • IgE Fc receptor subunit beta antibody
    • IGEL antibody
    • IGER antibody
    • IGHER antibody
    • LEU 16 antibody
    • LEU16 antibody
    • leukocyte surface antigen Leu 16 antibody
    • Leukocyte surface antigen Leu-16 antibody
    • Ly44 antibody
    • Membrane spanning 4 domains A1 antibody
    • Membrane spanning 4 domains subfamily A member 2 antibody
    • Membrane-spanning 4-domains subfamily A member 1 antibody
    • MS4A1 antibody
    • MS4A2 antibody
    • S7 antibody
    see all

Anti-CD20 antibody [B9E9] (FITC) 图像

  • Overlay histogram showing peripheral blood lymphocytes stained with ab1169 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1169, 0.06 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a FITC (1 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.

Anti-CD20 antibody [B9E9] (FITC) (ab1169)参考文献

ab1169 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. Although ab27856 has not been epitope mapped, the originator believes that it recognizes a region on the extracellular surface of CD20 because no permeabilization treatment is required prior to staining. For ab1169, th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"