概述

  • 产品名称Anti-CD20抗体
    参阅全部 CD20 一抗
  • 描述
    小鼠多克隆抗体to CD20
  • 经测试应用适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    CD20 (NP_068769.2, 1 a.a. ~ 297 a.a) full-length human protein.

  • 阳性对照
    • Human spleen membrane lysate Transfected 293T membrane lysate Human lymph node tissue CD20 expressing 293 cells

性能

应用

Our Abpromise guarantee covers the use of ab88247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab37355-Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能This protein may be involved in the regulation of B-cell activation and proliferation.
  • 组织特异性Expressed on B-cells.
  • 疾病相关Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • 序列相似性Belongs to the MS4A family.
  • 翻译后修饰Phosphorylated. Might be functionally regulated by protein kinase(s).
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APY antibody
    • ATOPY antibody
    • B lymphocyte antigen CD20 antibody
    • B Lymphocyte Cell Surface Antigen B1 antibody
    • B-lymphocyte antigen CD20 antibody
    • B-lymphocyte cell-surface antigen B1 antibody
    • B-lymphocyte surface antigen B1 antibody
    • B1 antibody
    • Bp 35 antibody
    • Bp35 antibody
    • CD 20 antibody
    • CD20 antibody
    • CD20 antigen antibody
    • CD20 receptor antibody
    • CD20_HUMAN antibody
    • CVID5 antibody
    • Fc epsilon receptor I beta chain antibody
    • Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody
    • FCER1B antibody
    • High affinity immunoglobulin epsilon receptor subunit beta antibody
    • IgE Fc receptor subunit beta antibody
    • IGEL antibody
    • IGER antibody
    • IGHER antibody
    • LEU 16 antibody
    • LEU16 antibody
    • leukocyte surface antigen Leu 16 antibody
    • Leukocyte surface antigen Leu-16 antibody
    • Ly44 antibody
    • Membrane spanning 4 domains A1 antibody
    • Membrane spanning 4 domains subfamily A member 2 antibody
    • Membrane-spanning 4-domains subfamily A member 1 antibody
    • MS4A1 antibody
    • MS4A2 antibody
    • S7 antibody
    see all

Anti-CD20 antibody 图像

  • Anti-CD20 antibody (ab88247) at 1 µg/ml + Human spleen at 25 µg

    Secondary
    Goat Anti-Mouse IgG at 1/2500 dilution

    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CD20 antibody (ab88247) at 1 µg/ml

    Lane 1 : CD20 transfected lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG at 1/2500 dilution

    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • ab88247, at 3µg/ml, staining human CD20 in lymph node, using Immunohistochemistry, Formalin/PFA-fixed paraffin embedded tissue.
  • FACS analysis of negative control 293 cells (Black) and CD20 expressing 293 cells (Green) using ab88247.

Anti-CD20 antibody (ab88247)参考文献

ab88247 has not yet been referenced specifically in any publications.

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