The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.25-0.5µg for 105-8 cells.
A final volume of 100 µl is recommended for staining the cell sample.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
Staining of normal Human peripheral blood cells with 0.25 µg of Mouse IgG1 ? Isotype Control Purified (open histogram) or 0.25 µg of ab95555 (filled histogram), followed by Anti-Mouse IgG PE. Cells in the lymphocyte gate were used for analysis.
Kap YS et al. A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates. J Histochem Cytochem57:1159-67 (2009).
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