The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.1µg for 106 cells. ab91362-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
功能CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
ab111896 at 0.1 µg/106 cells detecting CD2 in Fischer Rat lymph node cells by Flow Cytometry. 68% more of the cells were stained than in the control (FITC Mouse IgG2a). Cells from Lewis, Wistar, Brown Norway and Buffalo strains were also positive in this assay.