The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 10µl for 106 cells.
Use 10µl for 106 cells, or 100µl of whole blood.
ab18418-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
功能CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
FACS analysis of CD2 expression in human peripheral blood lymphocytes using biotin conjugated ab27994 at 10µl for 106 cells.
Anti-CD2 antibody [LT2] (Biotin) (ab27994)参考文献
This product has been referenced in:
Lin CW et al. CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood106:3567-74 (2005).
Read more (PubMed: 16046525) »
Beyers AD et al. Activation of T lymphocytes via monoclonal antibodies against rat cell surface antigens with particular reference to CD2 antigen. Immunol Rev111:59-77 (1989).
Read more (PubMed: 2576418) »