Anti-CD19抗体[CB19] (Phycoerythrin) (ab1168)

概述

  • 产品名称Anti-CD19抗体[CB19] (Phycoerythrin)
    参阅全部 CD19 一抗
  • 描述
    小鼠单克隆抗体[CB19] to CD19 (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human, Chimpanzee, Macaque Monkey, Rhesus monkey

性能

应用

Our Abpromise guarantee covers the use of ab1168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.

Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient. CD19 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10µl of CD19 (PE) is sufficient for labelling of 1x106 cells.



靶标

  • 功能Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
  • 疾病相关Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • 序列相似性Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • 翻译后修饰Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Antibody deficiency due to defect in CD19, included antibody
    • AW495831 antibody
    • B lymphocyte antigen CD19 antibody
    • B lymphocyte surface antigen B4 antibody
    • B-lymphocyte antigen CD19 antibody
    • B-lymphocyte surface antigen B4 antibody
    • B4 antibody
    • CD19 antibody
    • CD19 antigen antibody
    • CD19 molecule antibody
    • Cd19 protein antibody
    • CD19_HUMAN antibody
    • CVID3 antibody
    • Differentiation antigen CD19 antibody
    • Leu 12 antibody
    • Leu-12 antibody
    • Leu12 antibody
    • MGC109570 antibody
    • MGC12802 antibody
    • T-cell surface antigen Leu-12 antibody
    see all

Anti-CD19 antibody [CB19] (Phycoerythrin) 图像

  • ab1168 used in Flow Cytometry.
    Whole PBMC cells from Rhesus monkey were fixed with paraformaldehyde. The lymphocyte population was gated based on forward and side scatter properties. The cells within the gate were then analysis on 2 color dot plots with the CD19 (clone CB19) plotted on one axis and other lineage makers plotted on the other axis. CD3 and NKG2a positive population were negative for CD19 staining.All CD20 positive cells also stained positive for CD19. Addition analysis was carried out to verify that the monocyte and granulocyte populations were negative for staining with this CD19 antibody as is expected.

    ab1168 used undiluted.

    See Abreview

Anti-CD19 antibody [CB19] (Phycoerythrin) (ab1168)参考文献

This product has been referenced in:
  • Bradbury LE  et al. The CD19/CD21 signal transducing complex of human B lymphocytes includes the target of antiproliferative antibody-1 and Leu-13 molecules. J Immunol 149:2841-50 (1992). Read more (PubMed: 1383329) »
  • Carter RH & Fearon DT CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes. Science 256:105-7 (1992). Read more (PubMed: 1373518) »

See all 2 Publications for this product

Product Wall

Thank you for your inquiry.

I can confirm that the homology between human and Macaca fascicularis (Crab-eating macaque,Cynomolgus monkey) CD19 is 83%. (alignment of the full length sequences:P15391 andG7Q0T7)

This is border line, bu...

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Thank you for your inquiry.

I can confirm that the immunogen used to obtain clone CB19 is a human chronic lymphocytic leukemia cell line.

Wetherefore do nothave the specific immunogen sequence used to obtain this clone and to our kn...

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Thank you for contacting us.

Unfortunately, we don’t have any data to support the cross reactivity of this antibody with Cynomolgus monkey.

However, if you are interested in testing ab1168 in Cynomolgus monkey with Flow Cytome...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Rhesus monkey Cell (PBMC)
Specification PBMC
Preparation Cell harvesting/tissue preparation method: Whole blood staining and PBMC isolated on a ficoll spin.
Sample buffer: PBS with 2% FCS
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy The lymphocyte population was gated based on forward and side scatter properties. The cells within the gate were then analysis on 2 color dot plots with the CD19 (clone CB19) plotted on one axis and other lineage makers plotted on the other axis. CD3 and NKG2a positive population were negative for CD19 staining.All CD20 positive cells also stained positive for CD19. Addition analysis was carried out to verify that the monocyte and granulocyte populations were negative for staining with this CD19 antibody as is expected.
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Ken Rogers

Verified customer

提交于 Oct 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"