Anti-CD19抗体[A3-B1] (FITC) (ab91155)

概述

  • 产品名称
    Anti-CD19抗体[A3-B1] (FITC)
    参阅全部 CD19 一抗
  • 描述
    小鼠单克隆抗体[A3-B1] to CD19 (FITC)
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm
  • 特异性
    ab91155 is B lineage-specific and reacts with early B-cell precursors, pre-pre-B-cells, pre-B- cells, B-cells, intermediate B-cells, mature B-cells and some plasmacytoid cells. Plasma cells were found to be negative. This antibody does not react with other haemopoïetic cells. The antibody also reacts with pre-B-cell- lines, B lymphoblastoid cell-lines and Burkitt cell- lines, and with 50% of myeloma cell-lines. Virtually all non T-ALL, B-CLL and B-cell lymphomas were found to be positive, myeloma cells were found to be negative.
  • 经测试应用
    适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation (Human)

  • 阳性对照
    • Human B cells

性能

应用

Our Abpromise guarantee covers the use of ab91155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明
    Flow Cyt: Use 20µl per test.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
    • 疾病相关
      Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
    • 序列相似性
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • 翻译后修饰
      Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
    • 细胞定位
      Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Antibody deficiency due to defect in CD19, included antibody
      • AW495831 antibody
      • B lymphocyte antigen CD19 antibody
      • B lymphocyte surface antigen B4 antibody
      • B-lymphocyte antigen CD19 antibody
      • B-lymphocyte surface antigen B4 antibody
      • B4 antibody
      • CD19 antibody
      • CD19 antigen antibody
      • CD19 molecule antibody
      • Cd19 protein antibody
      • CD19_HUMAN antibody
      • CVID3 antibody
      • Differentiation antigen CD19 antibody
      • Leu 12 antibody
      • Leu-12 antibody
      • Leu12 antibody
      • MGC109570 antibody
      • MGC12802 antibody
      • T-cell surface antigen Leu-12 antibody
      see all

    图片

    • Human peripheral blood was labelled with ab91155 and analysed by flow cytometry.

    文献

    ab91155 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Monkey Cell (cryopreserved PBMC)
    Specification
    cryopreserved PBMC
    Preparation
    Cell harvesting/tissue preparation method: cells were thawed and washed in RPMI with 10% FCS plus Pen/Strep. Cells were counted using Trypan blue. Cells were transferred into FACS tubes and washed with DPBS. Antibodies were added and cells were stained for 30 min in the dark at RT. As positive control human PBMC were included.
    Sample buffer: DPBS during staining, DPBS plus 2% Formaldehyde
    Fixation
    Formaldehyde
    Permeabilization
    No
    Gating Strategy
    Cells were gated on Lymphocytes (SSC/FSC) followed by CD3/CD14 negative gating followed by CD19 positive, Isotype or CD20 as alternative control
    Username

    Abcam user community

    Verified customer

    提交于 Jan 06 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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