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RabMAb

Anti-CD18 (phospho S745)抗体[EP1288Y] (ab52920)

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概述

  • 产品名称
    Anti-CD18 (phospho S745)抗体[EP1288Y]
    参阅全部 CD18 一抗
  • 描述
    兔单克隆抗体[EP1288Y] to CD18 (phospho S745)
  • 宿主
    Rabbit
  • 特异性
    This antibody only detects CD18 phosphorylated on Serine 745.
  • 经测试应用
    适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC/IF or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    corresponding to Human CD18.

  • 阳性对照
    • Jurkat cell lysate or human tonsil tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: Raw 246.7
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab52920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
IP 1/50 - 1/100.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • 靶标

    • 功能
      Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
    • 疾病相关
      Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
    • 序列相似性
      Belongs to the integrin beta chain family.
      Contains 1 VWFA domain.
    • 翻译后修饰
      Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
    • 细胞定位
      Membrane.
    • Information by UniProt
    • 数据库链接
      • 别名
        • 95 subunit beta antibody
        • Beta 2 (antigen CD18 (p95) antibody
        • CD 18 antibody
        • CD18 antibody
        • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody
        • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody
        • Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody
        • Complement component 3 receptor 3 and 4 subunit antibody
        • Complement receptor C3 beta subunit antibody
        • Complement receptor C3 subunit beta antibody
        • Integrin beta 2 antibody
        • Integrin beta chain beta 2 antibody
        • Integrin beta-2 (ITGB2) antibody
        • Integrin beta-2 antibody
        • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody
        • ITB2_HUMAN antibody
        • ITGB2 antibody
        • LAD antibody
        • LCAMB antibody
        • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody
        • Leukocyte cell adhesion molecule CD18 antibody
        • LFA 1 antibody
        • LFA1 antibody
        • Lymphocyte function associated antigen 1 (LFA1) antibody
        • Lymphocyte function associated antigen 1 antibody
        • MAC 1 antibody
        • MAC1 antibody
        • Macrophage antigen 1 (mac1) beta antibody
        • MF17 antibody
        • MFI7 antibody
        • OTTHUMP00000115278 antibody
        • OTTHUMP00000115279 antibody
        • OTTHUMP00000115280 antibody
        • OTTHUMP00000115281 antibody
        • OTTHUMP00000115282 antibody
        see all

      图片

      • Immunoprecipitation - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)
        Immunoprecipitation - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)
        ab52920 (purified) at 1:50 dilution (2µg) immunoprecipitating CD18 in Jurkat whole cell lysate.
        Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
        Lane 2 (+): ab52920 & Jurkat whole cell lysate
        Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52920 in Jurkat whole cell lysate
        For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
        Blocking and diluting buffer: 5% NFDM/TBST.
      • Western blot - CD18 (phospho S745) antibody [EP1288Y] (ab52920)
        Western blot - CD18 (phospho S745) antibody [EP1288Y] (ab52920)
        All lanes : Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920) at 1/500 dilution (unpurified)

        Lane 1 : Jurkat cell lysate, untreated
        Lane 2 : Jurkat cell lysate, treated with AP.

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP labeled at 1/2000 dilution

        Predicted band size: 85 kDa
        Observed band size: 85 kDa

      文献

      ab52920 has not yet been referenced specifically in any publications.

      发表研究结果有使用 ab52920？请让我们知道，以便我们可以引用本数据表中的参考文章。

      客户评价及客户问答

      提交评价 提交问题

      8 reviews or Q&A

      10
      Votes: Highest First

      vielen Dank für Ihre Antwort. Unsere MTA hat mir allerdings eine andere Lot-Nr. aufgeschrieben: GR31039-4. Gibt es sehr große Unterschiede zwischen den Konzentrationen der Lots? Oder könnten Sie bitte die Konzentartion dieses Lots nochm...

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      Vielen Dank für Ihre Email.

      Beide Nummern (1213063 und GR31039-4) beschreiben dasselbe Lot.

      Ich kann bestätigen, dass die Konzentration für dieses Lot 1.283 mg/mL ist.

      ich hoffe, diese Information ist hilfre...

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      Konzentration von ab52920?

      Vielen Dank für Ihren Anruf.

      Ich freue mich nun die Konzentration von Lot 1213063 bestätigen zu können:

      1.283 mg/mL.

      Ich hoffe, dies wird Ihnen beim Einstellen der Isotypkontrolle weiterhelfen. Bitte beachte...

      Read More

      Inquiry: I plan to stain a section of human periodontal tissue for CD18 using IF. I decided to pick an antibody that can be used later for IHC of other human samples if needed. Looking online, it appears that ab52920 is a suitabel product. However, I n...

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      Thank you for contacting us.

      I advise not buying ab52920 if you want to stain all CD18. The antibody is raised against a phosphorylated site on CD18, and will only stain CD18 that is phosphorylated at that site, not total CD18.

      Th...

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      The customer kindly phoned today because neither of the products in this shipment was giving real signal in IHC-P.

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has...

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      I would like to know what is the concentration (or amount in the tube) of
      the following antibodies:
      ab52920
      ab63388

      Thank you for contacting Abcam.

      For ab63388 you get 100 µg at 1mg/ml. For ab52920 you get 100ul butthe concentration varies slightly from batch to batch and so if you could let me know the lot number that you currently have, I will be h...

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      How about ab63388? Is this antibody also applicable for immunoblot. Kind regards,

      Thank you for contacting us. ab63388 hasn't been tested in WB so we can’t confirm the suitability of this antibody due to lack of tested data. However this does not mean this cannot be used in WB this only means we have not tested it. If you are ...

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      Actually, we don’t know which amino acid(s) will be phosphorylated in our experimental setting. I guess the T758 position. Is this antibody applicable for immunoblot? Kind regards,

      The ab52920 can be used in Western Blot, Immunohistochemistry (paraffin embedded tissue sections, immunoprecipitation and Immunocytochemistry / Immunofluorescence. I hope this will help!

      Hi, do you antibody against phorylated beta2 integrin? Thanks,

      Thank you for contacting us. Could you let us know the phosphorylated amino acid you are interested in? The integrin beta 2 shows phosphorylation at Serine 745, 756 and at Threonine 758, 759 and 760. The antibodies we have are ab52920 and ab63388 I hop...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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