概述
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产品名称Anti-CD18 (phospho S745)抗体[EP1288Y]
参阅全部 CD18 一抗 -
描述兔单克隆抗体[EP1288Y] to CD18 (phospho S745)
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宿主Rabbit
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特异性This antibody only detects CD18 phosphorylated on Serine 745.
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经测试应用适用于: WB, IPmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性与反应: Mouse, Rat, Human
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免疫原
Synthetic peptide within Human CD18. The exact sequence is proprietary.
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阳性对照
- Jurkat cell lysate or human tonsil tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: Raw 246.7
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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存储溶液pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度Protein A purified
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克隆单克隆
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克隆编号EP1288Y
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同种型IgG
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研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab52920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). | |
| IP | 1/50 - 1/100. |
靶标
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功能Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
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疾病相关Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
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序列相似性Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
翻译后修饰Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
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细胞定位Membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 3689 Human
- Entrez Gene: 16414 Mouse
- Entrez Gene: 309684 Rat
- Omim: 600065 Human
- SwissProt: P05107 Human
- SwissProt: P11835 Mouse
- Unigene: 375957 Human
- Unigene: 1137 Mouse
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别名
- 95 subunit beta antibody
- Beta 2 (antigen CD18 (p95) antibody
- CD 18 antibody
see all
图片
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![Immunoprecipitation - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)](http://www.abcam.cn/ps/products/52/ab52920/Images/ab52920-5-ab52920IP1.jpg)
Immunoprecipitation - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)ab52920 (purified) at 1:50 dilution (2µg) immunoprecipitating CD18 in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab52920 & Jurkat whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52920 in Jurkat whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)](http://www.abcam.cn/ps/products/52/ab52920/Images/ab52920_1.jpg)
Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)All lanes : Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920) at 1/500 dilution (unpurified)
Lane 1 : Un-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : AP-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa
文献
ab52920 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"