概述
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产品名称Anti-CD18 (phospho S745)抗体[EP1288Y]
参阅全部 CD18 一抗 -
描述兔单克隆抗体[EP1288Y] to CD18 (phospho S745)
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特异性This antibody only detects CD18 phosphorylated on Serine 745.
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经测试应用适用于: WB, IP, IHC-P, ICC/IF, Flow Cytmore details
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种属反应性与反应: Mouse, Rat, Human
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免疫原
Synthetic phospho-peptide corresponding to residues surrounding Ser745 of human CD18.
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阳性对照
- Jurkat cell lysate or human tonsil tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: Raw 246.7
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常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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纯度Tissue culture supernatant
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克隆单克隆
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克隆编号EP1288Y
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同种型IgG
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研究领域
相关产品
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Positive Controls
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Related Products
应用
Our Abpromise guarantee covers the use of ab52920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). | |
| IP | 1/100. | |
| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | 1/250 - 1/500. | |
| Flow Cyt | 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
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疾病相关Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
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序列相似性Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
翻译后修饰Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
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细胞定位Membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 3689 Human
- Entrez Gene: 16414 Mouse
- Entrez Gene: 309684 Rat
- Omim: 600065 Human
- SwissProt: P05107 Human
- SwissProt: P11835 Mouse
- Unigene: 375957 Human
- Unigene: 1137 Mouse
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别名
- 95 subunit beta antibody
- Beta 2 (antigen CD18 (p95) antibody
- CD 18 antibody
see all
Anti-CD18 (phospho S745) antibody [EP1288Y] 图像
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![Immunocytochemistry/ Immunofluorescence - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)](http://www.abcam.cn/ps/products/52/ab52920/Images/ab52920-172115-ab52920gr310394edited.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)ICC/IF image of ab52920 stained Raw 246.7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab52920 at 1/1000 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Western blot - CD18 (phospho S745) antibody [EP1288Y] (ab52920)](http://www.abcam.cn/ps/products/52/ab52920/Images/ab52920_1.jpg)
Western blot - CD18 (phospho S745) antibody [EP1288Y] (ab52920)All lanes : Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920) at 1/500 dilution
Lane 1 : Jurkat cell lysate, untreated
Lane 2 : Jurkat cell lysate, treated with AP.
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size : 85 kDa
Observed band size : 85 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)](http://www.abcam.cn/ps/products/52/ab52920/Images/ab52920_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)Ab52920 (1:250) staining human CD18 in human tonsil tissue by immunohistochemistry using paraffin embedded tissue. -
![Flow Cytometry - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)](http://www.abcam.cn/ps/products/52/ab52920/Images/ab52920-4-ab52920FC.jpg)
Flow Cytometry - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)Human peripheral blood lymphocytes stained with ab52920 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab52920, 1/1000 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.
Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)参考文献
ab52920 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"