重组Anti-CD18 (phospho S745)抗体[EP1288Y] (ab52920)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1288Y] to CD18 (phospho S745)
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CD18 (phospho S745)抗体[EP1288Y]
参阅全部 CD18 一抗 -
描述
兔单克隆抗体[EP1288Y] to CD18 (phospho S745) -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human CD18 (phospho S745). The exact sequence is proprietary.
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阳性对照
- WB: Jurkat, NIH/3T3 and PC-12 cell lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: Raw 246.7
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1288Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52920于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
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IP |
1/50 - 1/100.
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). |
IP
1/50 - 1/100. |
靶标
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功能
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. -
疾病相关
Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions. -
序列相似性
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
翻译后修饰
Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3689 Human
- Entrez Gene: 16414 Mouse
- Entrez Gene: 309684 Rat
- Omim: 600065 Human
- SwissProt: P05107 Human
- SwissProt: P11835 Mouse
- Unigene: 375957 Human
- Unigene: 1137 Mouse
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别名
- 95 subunit beta antibody
- Beta 2 (antigen CD18 (p95) antibody
- CD 18 antibody
see all
图片
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All lanes : Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920) at 0.9 µg/ml (purified)
Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates 15ug. Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 85 kDa
Blocking and diluting buffer: 5% NFDM/TBST -
All lanes : Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920) at 0.9 µg/ml (purified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates. Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 85 kDa
Blocking and diluting buffer: 5% NFDM/TBST -
All lanes : Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920) at 0.9 µg/ml (purified)
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates. Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 85 kDa
Blocking and diluting buffer: 5% NFDM/TBST -
ab52920 (purified) at 1:50 dilution (2µg) immunoprecipitating CD18 in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab52920 & Jurkat whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52920 in Jurkat whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
All lanes : Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920) at 1/500 dilution (unpurified)
Lane 1 : Un-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : AP-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab52920 被引用在 2 文献中.
- Han X et al. Complement receptor 3 mediates Aspergillus fumigatus internalization into alveolar epithelial cells with the increase of intracellular phosphatidic acid by activating FAK. Virulence 12:1980-1996 (2021). PubMed: 34338598
- Ye Y et al. QiShenYiQi Inhibits Tissue Plasminogen Activator-Induced Brain Edema and Hemorrhage after Ischemic Stroke in Mice. Front Pharmacol 12:759027 (2021). PubMed: 35095486