Anti-CD18抗体[MEM-48] - Low endotoxin，Azide free (ab46700)

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概述

  • 产品名称
    Anti-CD18抗体[MEM-48] - Low endotoxin，Azide free
    参阅全部 CD18 一抗
  • 描述
    小鼠单克隆抗体[MEM-48] to CD18 - Low endotoxin，Azide free
  • 宿主
    Mouse
  • 特异性
    The antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin ß2 subunit; ß2 integrin).
  • 经测试应用
    适用于: Flow Cyt, IP, WB, IHC-Fr, Functional Studiesmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Leucocytes of a patient suffering from a LGL-type leukaemia.

  • 阳性对照
    • WB: Kg-1a human leukemia cell lysate
  • 常规说明
    Preserved by filter sterilization

性能

应用

Our Abpromise guarantee covers the use of ab46700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IP Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Use under non reducing condition. Predicted molecular weight: 85 kDa. Use under non reducing conditions. Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS. SDS-PAGE (6% separating gel, 4% stacking gel).
IHC-Fr Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. This antibody is useful for studies of cell-cell and cell-matrix adhesion. It induces high-affinity conformation of LFA-1 complex.

靶标

  • 功能
    Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
  • 疾病相关
    Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
  • 序列相似性
    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • 翻译后修饰
    Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
    • 别名
      • 95 subunit beta antibody
      • Beta 2 (antigen CD18 (p95) antibody
      • CD 18 antibody
      • CD18 antibody
      • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody
      • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody
      • Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody
      • Complement component 3 receptor 3 and 4 subunit antibody
      • Complement receptor C3 beta subunit antibody
      • Complement receptor C3 subunit beta antibody
      • Integrin beta 2 antibody
      • Integrin beta chain beta 2 antibody
      • Integrin beta-2 (ITGB2) antibody
      • Integrin beta-2 antibody
      • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody
      • ITB2_HUMAN antibody
      • ITGB2 antibody
      • LAD antibody
      • LCAMB antibody
      • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody
      • Leukocyte cell adhesion molecule CD18 antibody
      • LFA 1 antibody
      • LFA1 antibody
      • Lymphocyte function associated antigen 1 (LFA1) antibody
      • Lymphocyte function associated antigen 1 antibody
      • MAC 1 antibody
      • MAC1 antibody
      • Macrophage antigen 1 (mac1) beta antibody
      • MF17 antibody
      • MFI7 antibody
      • OTTHUMP00000115278 antibody
      • OTTHUMP00000115279 antibody
      • OTTHUMP00000115280 antibody
      • OTTHUMP00000115281 antibody
      • OTTHUMP00000115282 antibody
      see all

    图片

    • Flow Cytometry - Anti-CD18 antibody [MEM-48] - Low endotoxin, Azide free (ab46700)
      Flow Cytometry - Anti-CD18 antibody [MEM-48] - Low endotoxin, Azide free (ab46700)

      Overlay histogram showing peripheral blood lymphocytes stained with ab46700 (red line). The cells were incubated with the antibody (ab46700, 1µg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/200 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.

    文献

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