概述

  • 产品名称
    Anti-CD18抗体[MEM-48]
    参阅全部 CD18 一抗
  • 描述
    小鼠单克隆抗体[MEM-48] to CD18
  • 特异性
    Human CD18 antigen. This antibody recognizes all three leucocyte integrins.
  • 经测试应用
    适用于: ICC/IF, IHC-P, Flow Cyt, IHC-Fr, IP, WB, Functional Studiesmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation (Human). (Leucocytes of a patient suffering from a LGL-type leukaemia).

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. We would recommend antibody ab8220 as a replacement.

性能

应用

Our Abpromise guarantee covers the use of ab657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 24991932
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 0.1-1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use a concentration of 10 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Use under non reducing condition.

BSA is recommended for blocking.

Functional Studies Use at an assay dependent concentration. The antibody induces high-affinity conformation of LFA-1 complex.

靶标

  • 功能
    Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
  • 疾病相关
    Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
  • 序列相似性
    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • 翻译后修饰
    Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 95 subunit beta antibody
    • Beta 2 (antigen CD18 (p95) antibody
    • CD 18 antibody
    • CD18 antibody
    • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody
    • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody
    • Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody
    • Complement component 3 receptor 3 and 4 subunit antibody
    • Complement receptor C3 beta subunit antibody
    • Complement receptor C3 subunit beta antibody
    • Integrin beta 2 antibody
    • Integrin beta chain beta 2 antibody
    • Integrin beta-2 (ITGB2) antibody
    • Integrin beta-2 antibody
    • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody
    • ITB2_HUMAN antibody
    • ITGB2 antibody
    • LAD antibody
    • LCAMB antibody
    • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody
    • Leukocyte cell adhesion molecule CD18 antibody
    • LFA 1 antibody
    • LFA1 antibody
    • Lymphocyte function associated antigen 1 (LFA1) antibody
    • Lymphocyte function associated antigen 1 antibody
    • MAC 1 antibody
    • MAC1 antibody
    • Macrophage antigen 1 (mac1) beta antibody
    • MF17 antibody
    • MFI7 antibody
    • OTTHUMP00000115278 antibody
    • OTTHUMP00000115279 antibody
    • OTTHUMP00000115280 antibody
    • OTTHUMP00000115281 antibody
    • OTTHUMP00000115282 antibody
    see all

Anti-CD18 antibody [MEM-48] 图像

  • Human peripheral blood lymphocytes stained with ab657 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22ºC. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37ºC. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4ºC. Cells were then incubated with the antibody (ab657, 1μg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

Anti-CD18 antibody [MEM-48] (ab657)参考文献

This product has been referenced in:
  • Xiang Y  et al. Analysis on the relevance of asthma susceptibility with the alteration of integrin ß 4 expression. PLoS One 9:e95533 (2014). WB ; Human . Read more (PubMed: 24740264) »
  • Giese S & Marsh M Tetherin can restrict cell-free and cell-cell transmission of HIV from primary macrophages to T cells. PLoS Pathog 10:e1004189 (2014). ICC/IF . Read more (PubMed: 24991932) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell lysate - whole cell (HEK293 cells overexpressing cd18/cd11b)
Specification
HEK293 cells overexpressing cd18/cd11b
Permeabilization
No
Username

Abcam user community

Verified customer

提交于 Aug 18 2005

Thank you for your enquiry. These two antibodies were characterized using HPB-ALL or Jurkat T cells. Triplet of zones on NON-REDUCING lysates is usually observed (78-96 kDa). If you have any additional questins, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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