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Our Abpromise guarantee covers the use of ab119830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 85 kDa.|
|IP||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.|
|Blocking||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use a concentration of 1 µg/ml. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
ICC/IF image of CD18 staining on RAW264.7 cells using ab119830 (1:300). The cells were fixed using formaldehyde and later permeabilized using 0.2% Triton X-100. The cells were blocked using 10% goat serum for 1 hour at 20°C. ab119830 was diluted to 1:300 and incubated with the cells for 1 hour at 20°C. The secondary antibody used was Anti-rat goat polyclonal conjugated to Alexa Fluor 647 (1:350).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"