Anti-CD18抗体[2E6] (ab119347)
Key features and details
- Hamster monoclonal [2E6] to CD18
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-CD18抗体[2E6]
参阅全部 CD18 一抗 -
描述
仓鼠单克隆抗体[2E6] to CD18 -
宿主
Hamster -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Mouse -
免疫原
Full length protein corresponding to Human CD18 aa 1-100. Native protein
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阳性对照
- Flow Cyt: Recombinant Human CD18 protein (ab114283) can be used as a positive control in WB.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. -
存储溶液
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
2E6 -
同种型
IgG -
研究领域
相关产品
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119347于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use at an assay dependent concentration.
ab18474 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
说明 |
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Flow Cyt
Use at an assay dependent concentration. ab18474 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. -
疾病相关
Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions. -
序列相似性
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
翻译后修饰
Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 16414 Mouse
- SwissProt: P11835 Mouse
- Unigene: 1137 Mouse
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别名
- 95 subunit beta antibody
- Beta 2 (antigen CD18 (p95) antibody
- CD 18 antibody
see all
图片
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Flow cytometry analysis of CD18 in mouse splenocytes (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (ab119347) at a dilution of 2 ug/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated secondary antibody for 30 min at 4ºC in the dark. FACS analysis was performed using 400 ul of cell buffer.
数据表及文件
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Datasheet download
文献 (4)
ab119347 被引用在 4 文献中.
- Tasaka S et al. Very late antigen-4 in CD18-independent neutrophil emigration during acute bacterial pneumonia in mice. Am J Respir Crit Care Med 166:53-60 (2002). Blocking ; Mouse . PubMed: 12091171
- Poon BY et al. alpha(4)-integrin mediates neutrophil-induced free radical injury to cardiac myocytes. J Cell Biol 152:857-66 (2001). Blocking ; Mouse . PubMed: 11238444
- Lawson JA et al. The hepatic inflammatory response after acetaminophen overdose: role of neutrophils. Toxicol Sci 54:509-16 (2000). Blocking ; Mouse . PubMed: 10774834
- Poon BY et al. Emigrated neutrophils regulate ventricular contractility via alpha4 integrin. Circ Res 84:1245-51 (1999). Blocking, Flow Cyt ; Mouse . PubMed: 10364561