Anti-CD177抗体[MEM-166] (ab8092)

概述

  • 产品名称Anti-CD177抗体[MEM-166]
    参阅全部 CD177 一抗
  • 描述
    小鼠单克隆抗体[MEM-166] to CD177
  • 特异性CD177 antigen (human)
  • 经测试应用适用于: ICC/IF, IHC-P, Flow Cyt, IP, WBmore details
  • 种属反应性
    与反应: Human, Monkey, Rhesus monkey
  • 免疫原

    Human granulocytes

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from ascites using protein A affinity chromatography.
  • 克隆单克隆
  • 克隆编号MEM-166
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
IHC-P Use at an assay dependent concentration. PubMed: 23599270
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Use under non reducing condition.

靶标

  • 组织特异性Highly expressed in normal bone marrow and weakly expressed in fetal liver. Expressed on neutrophils. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET).
  • 序列相似性Contains 2 UPAR/Ly6 domains.
  • 翻译后修饰N-glycosylated.
    A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD177 antibody
    • CD177 antigen antibody
    • CD177 molecule antibody
    • CD177_HUMAN antibody
    • HNA-2a antibody
    • HNA2A antibody
    • Human neutrophil alloantigen 2a antibody
    • NB1 antibody
    • NB1 glycoprotein antibody
    • NB1 GP antibody
    • Polycythemia rubra vera 1 antibody
    • Polycythemia rubra vera protein 1 antibody
    • PRV-1 antibody
    • PRV1 antibody
    see all

Anti-CD177 antibody [MEM-166] 图像

  • ab8092 at 1/200 staining human eripheral blood derived CD34+ cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 24 hours at 4°C. A FITC conjugated goat anti-mouse IgG was used as the secondary. The cells wered counter stained with DAPI.

    See Abreview

Anti-CD177 antibody [MEM-166] (ab8092)参考文献

This product has been referenced in:
  • Hu N  et al. Differential Expression of Granulopoiesis Related Genes in Neutrophil Subsets Distinguished by Membrane Expression of CD177. PLoS One 9:e99671 (2014). WB, Flow Cyt ; Human . Read more (PubMed: 24926686) »
  • Stepensky P  et al. The Thr224Asn mutation in the VPS45 gene is associated with the congenital neutropenia and primary myelofibrosis of infancy. Blood 121:5078-87 (2013). IHC-P ; Human . Read more (PubMed: 23599270) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us.

According to our datasheet, the only thing which needs consideration is the fact that this antibody will detect under non-reducing conditions only. This would mean that you would have to leave out the reducing agen...

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Thank you for contacting us regarding testing of AB8092 on rabbit tissues.

I have had the opportunity to BLAST the immunogen against rabbit on both Uniprot and NCBI Unfortunately, the best homologies that I have found are have identities of ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Peripheral blood derived CD34+ cells)
Specification Peripheral blood derived CD34+ cells
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

提交于 Jun 26 2007

Thank you for your enquiry. I am sorry to hear that you have been having difficulty with this antibody. You have tried many of the suggestions that I would make such as applying this antibody at 4oC overnight using BSA as a blocking agent. You are a...

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The epitope recognised by this mouse monoclonal antibody has not been mapped. It is supplied at a concentration of 1 mg/ml so I'd recommend a 1:1000 starting dilution for WB. The antibody has been shown to be suitbale for use in WB, IP and flow cytomet...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"