The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1 - 1/10.
Neat - 1/10. Use 10µl to label 106 cells in 100µl.
ab136585 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Function: Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
Tissue specificity: Found on monocytes, neutrophils and eosinophil platelets.
Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
PTM: Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.
CD32: Type I membrane protein.
CD16: Attached to the membrane by a GPI anchor. Exists also as a soluble receptor, produced by a proteolytic cleavage.
Olme CH et al. Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia. Leuk Res37:1374-82 (2013).
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