The details of the immunogen for this antibody are not available.
表位This antibody recognizes a conformational epitope formed by Fc gamma II and Fc gamma III receptors.
常规说明This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
This antibody besides identifying Fcgamma R+ cells, has been used to block Fc receptor binding of IgG, Fc mediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on Fc gamma R for parasitic invasion.
Primary antibody说明This antibody besides identifying Fcgamma R+ cells, has been used to block Fc receptor binding of IgG, Fc mediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on Fc gamma R for parasitic invasion.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.2µg for 106 cells. ab154456-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
相关性In mouse only the transmembrane form of CD16 is reported to exist, which is expressed on macrophages, NK cells, granulocytes, myeloid precursors, and subpopulations of T lymphocytes. CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes. The lymphocyte Fc gamma receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta. The Fc gamma type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios. The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression.
细胞定位CD32: Type I membrane protein.
CD16: Attached to the membrane by a GPI anchor. Exists also as a soluble receptor, produced by a proteolytic cleavage.