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RabMAb

Anti-CD168抗体[EPR4055] (ab108339)

概述

  • 产品名称
    Anti-CD168抗体[EPR4055]
    参阅全部 CD168 一抗
  • 描述
    兔单克隆抗体[EPR4055] to CD168
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within Human CD168.

  • 阳性对照
    • T47-D MCF-7, SKBR-3 and LnCaP cell lysate Paraffin-embedded human breast carcinoma tissue Paraffin-embedded human testis tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab108339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 84 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明
    Is unsuitable for ICC/IF.
  • 靶标

    • 功能
      Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including the focal adhesion kinase occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity.
    • 组织特异性
      Expressed in breast cancer cell lines and in normal breast tissue.
    • 细胞定位
      Cell surface. Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD168 antibody
      • CD168 antigen antibody
      • HMMR antibody
      • HMMR_HUMAN antibody
      • Hyaluronan mediated motility receptor antibody
      • Hyaluronan-mediated motility receptor (RHAMM) antibody
      • IHABP antibody
      • Intracellular hyaluronic acid-binding protein antibody
      • MGC119494 antibody
      • MGC119495 antibody
      • OTTHUMP00000196920 antibody
      • Receptor for hyaluronan-mediated motility antibody
      • RHAMM antibody
      see all

    图片



    • Predicted band size : 84 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: Empty knockout  HAP1 whole cell lysate (20 µg)
      Lane 3: CD168 whole cell lysate (20 µg)              

      Lanes 1 - 3: Merged signal (red and green). Green - ab108339 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab108339 was shown to specifically react with CD168 when CD168 knockout samples were used. Wild-type and Empty knockout samples were subjected to SDS-PAGE.  Ab108339 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • ab108339, at 1/100, staining CD168 in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.
    • All lanes : Anti-CD168 antibody [EPR4055] (ab108339) at 1/1000 dilution

      Lane 1 : T47-D cell lysate
      Lane 2 : MCF-7 cell lysate
      Lane 3 : SKBR-3 cell lysate
      Lane 4 : LnCaP cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 84 kDa
    • ab108339, at 1/100, staining CD168 in paraffin-embedded human testis tissue by Immunohistochemistry.
    • ab108339 showing positive staining in Normal thymus tissue.

    • ab108339 showing positive staining in Normal stomach tissue.

    • ab108339 showing negative staining in Normal brain tissue.

    • ab108339 showing positive staining in Colonic adenocarcinoma tissue.

    • ab108339 showing negative staining in Normal liver tissue.

    • ab108339 showing positive staining in Normal tonsil tissue.

    文献

    This product has been referenced in:
    • Wang K & Zhang T Prognostic significance of CD168 overexpression in colorectal cancer. Oncol Lett 12:2555-2559 (2016). IHC ; Human . Read more (PubMed: 27698827) »
    • Wang D  et al. Expression of the receptor for hyaluronic acid mediated motility (RHAMM) is associated with poor prognosis and metastasis in non-small cell lung carcinoma. Oncotarget 7:39957-39969 (2016). WB, IHC . Read more (PubMed: 27220886) »

    See all 3 Publications for this product

    客户评价及客户问答

    For ab124729 lot GR82264-8, the concentration is 1.797 mg/ml.

    The current lot we have for sale for ab108339 is lot GR106937-4 and that is 0.193 mg/ml.

    There should not be concerns about permeabilizing cells because as soon as they are fixed with parafomraldehyde, they are essentially permeabilized. The CD168 antigen is a non-integral cell surface hyaluronan receptor that is readily accessable to the ...

    Read More

    A link below is our Flow Cytometry protocol, which we use routinely for in-house testing.
    http://www.epitomics.com/products/support/fc
    Our protocol is for intracellular staining and we always fix the cells with 2% paraformaldehyde.

    Thank you for your email.
    I can confirm that ab108339 (Anti-CD168 antibody) would recognize all 4 isoforms of CD168/RHAMM.
    If you need any further assistance in the future, please do not hesitate to contact me.

    Thank you for contacting Abcam earlier today.

    Please find attached the IHC-P protocol for both ab108339 & ab124729.

    If there is anything else I can help you with, please let me know.

    Thank you for contacting us.
    The target CD168 is a cell surface protein. There no need for cell permeabilization and fixation (do not wait longer than an hour). Cell fixatiion is advised if there is a wait longer than an hour between analysis. Avo...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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