重组Anti-CD168抗体[EPR4054] (ab124729)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4054] to CD168
- Suitable for: WB, IP, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CD168抗体[EPR4054]
参阅全部 CD168 一抗 -
描述
兔单克隆抗体[EPR4054] to CD168 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human CD168 aa 1-100. The exact sequence is proprietary.
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阳性对照
- Lysates of: MDA-MB-435, LnCaP, T47-D, RAW264.7, PC-12. Human testis tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.23 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4054 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124729于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/1000. Predicted molecular weight: 84 kDa.
For unpurified use at 1/1000 - 1/10000. The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples. |
IP |
1/10 - 1/100.
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IHC-P |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For unpurified use at 1/50 - 1/100. Antigen retrieval is recommended |
说明 |
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WB
1/1000. Predicted molecular weight: 84 kDa. For unpurified use at 1/1000 - 1/10000. The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples. |
IP
1/10 - 1/100. |
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified use at 1/50 - 1/100. Antigen retrieval is recommended |
靶标
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功能
Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including the focal adhesion kinase occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity. -
组织特异性
Expressed in breast cancer cell lines and in normal breast tissue. -
细胞定位
Cell surface. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 3161 Human
- Entrez Gene: 15366 Mouse
- Entrez Gene: 25460 Rat
- Omim: 600936 Human
- SwissProt: O75330 Human
- SwissProt: Q00547 Mouse
- SwissProt: P97779 Rat
- Unigene: 728200 Human
see all -
别名
- CD168 antibody
- CD168 antigen antibody
- HMMR antibody
see all
图片
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Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Empty
Lane 3: CD168 knockout HAP1 whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab124729 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab124729 was shown to specifically react with CD168 when CD168 knockout samples were used. Wild-type and CD168 knockout samples were subjected to SDS-PAGE. Ab124729 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric carcinoma tissue sections labeling CD168 with purified ab124729 at 1:250 dilution (7.7 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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ab124729 (purified) at 1:100 dilution (2ug) immunoprecipitating CD168 in MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate.
Lane 1 (input): MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab124729 & MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124729 in MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Immunohistochemical analysis of paraffin-embedded human testis tissue using unpurified ab124729 at 1/50 - 1/100 dilution
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All lanes : Anti-CD168 antibody [EPR4054] (ab124729) at 1/1000 dilution (purified)
Lane 1 : MDA-MB-435 (Human mammary gland ductal carcinoma melanocyte) whole cell lysates at 20 µg
Lane 2 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 15 µg
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysates at 15 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 84 kDa5% NFDM/TBST
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All lanes : Anti-CD168 antibody [EPR4054] (ab124729)
Lane 1 : MDA-MB-435 cell lysate
Lane 2 : LnCaP cell lysate
Lane 3 : T47-D cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 84 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab124729 被引用在 13 文献中.
- Lin A et al. High levels of truncated RHAMM cooperate with dysfunctional p53 to accelerate the progression of pancreatic cancer. Cancer Lett 514:79-89 (2021). PubMed: 34044069
- Mejia J et al. A replicating stem-like cell that contributes to bone morphogenetic protein 2-induced heterotopic bone formation. Stem Cells Transl Med 10:623-635 (2021). PubMed: 33245845
- Lu T et al. High Expression of Hyaluronan-Mediated Motility Receptor Predicts Adverse Outcomes: A Potential Therapeutic Target for Head and Neck Squamous Cell Carcinoma. Front Oncol 11:608842 (2021). PubMed: 33763352
- Li Z et al. Identification of HMMR as a prognostic biomarker for patients with lung adenocarcinoma via integrated bioinformatics analysis. PeerJ 9:e12624 (2021). PubMed: 35036134
- Ye S et al. TGFß and Hippo Pathways Cooperate to Enhance Sarcomagenesis and Metastasis through the Hyaluronan-Mediated Motility Receptor (HMMR). Mol Cancer Res 18:560-573 (2020). PubMed: 31988250