重组
RabMAb

Anti-CD166抗体[EPR2759(2)] (ab109215)

概述

  • 产品名称
    Anti-CD166抗体[EPR2759(2)]
    参阅全部 CD166 一抗
  • 描述
    兔单克隆抗体[EPR2759(2)] to CD166
  • 经测试应用
    适用于: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD166.

  • 阳性对照
    • WB: SH-SY5Y, HuT-78, HT-1080 and Daudi cell lysates. Mouse and rat brain tissue lysates. IHC-P: Human liver and prostatic adenocarcinoma tissues. ICC/IF: THP-1 cells. Flow Cyt: HuT-78 cells. IP: SH-SY5Y cells.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab109215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/20000. Detects a band of approximately 100-105 kDa (predicted molecular weight: 65 kDa).

For unpurified use at 1/1000 - 1/10000.

IP 1/30.

For unpurified use at 1/10 - 1/100.

IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified use at 1/100 - 1/250.

Flow Cyt 1/90. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/50 - 1/250.

靶标

  • 功能
    Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.
  • 组织特异性
    Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines.
  • 序列相似性
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.
  • 结构域
    The CD6 binding site is located in the N-terminal Ig-like domain.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activated leukocyte cell adhesion molecule antibody
    • ALCAM antibody
    • ALCAM protein antibody
    • CD 166 antibody
    • CD166 antibody
    • CD166 antigen antibody
    • CD166_HUMAN antibody
    • FLJ3851 antibody
    • FLJ38514 antibody
    • MEMD antibody
    • MGC71733 antibody
    see all

图片

  • All lanes : Anti-CD166 antibody [EPR2759(2)] (ab109215) at 1/10000 dilution (purified)

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : HuT-78 cell lysate
    Lane 3 : HT-1080 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 65 kDa
    Observed band size : 100-105 kDa (why is the actual band size different from the predicted?)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling CD166 with purified ab109215 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/Immunofluorescence analysis of THP-1 (human acute monocytic leukemia) cells labelling CD166 (green) with purified ab109215 at 1/250. Cells were fixed with 100% methanol. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as a nuclear counterstain.  

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • All lanes : Anti-CD166 antibody [EPR2759(2)] (ab109215) at 1/10000 dilution (purified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 65 kDa
    Observed band size : 100-105 kDa (why is the actual band size different from the predicted?)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling CD166 with unpurified ab109215 at 1/100.

  • All lanes : Anti-CD166 antibody [EPR2759(2)] (ab109215) at 1/1000 dilution (unpurified)

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : HuT-78 cell lysate
    Lane 3 : HT-1080 cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 65 kDa
    Observed band size : 100-105 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic adenocarcinoma tissue labelling CD166 with unpurified ab109215 at 1/100.

  • Flow Cytometry analysis of HuT-78 cells labelling CD166 with purified ab109215 at 1/90 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • ab109215 (purified) at 1/30 immunoprecipitating CD166 in SH-SY5Y cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

文献

This product has been referenced in:
  • Dorfmueller S  et al. Isolation of a recombinant antibody specific for a surface marker of the corneal endothelium by phage display. Sci Rep 6:21661 (2016). Read more (PubMed: 26902886) »
  • Zhou RP  et al. Shp2 regulates chlorogenic acid-induced proliferation and adipogenic differentiation of bone marrow-derived mesenchymal stem cells in adipogenesis. Mol Med Rep 11:4489-95 (2015). Read more (PubMed: 25634525) »

See all 5 Publications for this product

客户评价及客户问答

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (melanoma 4L cell line)
Total protein in input
1000 µg
Immuno-precipitation step
Protein G
Specification
melanoma 4L cell line
Username

Abcam user community

Verified customer

提交于 May 12 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (Melanoma cells 4L)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Melanoma cells 4L
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 12 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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