Anti-CD163抗体[RM3/1] (ab17051)

概述

  • 产品名称Anti-CD163抗体[RM3/1]
    参阅全部 CD163 一抗
  • 描述
    小鼠单克隆抗体[RM3/1] to CD163
  • 经测试应用适用于: WB, Flow Cyt, IP, Functional Studies, ICC/IF, IHC-Frmore details
    不适用于: IHC-G or IHC-P
  • 种属反应性
    与反应: Human, Monkey
    不与反应: Rat, Guinea pig, Pig
  • 免疫原

    Human monocytes (epitope recognized SRCR domain 9).

  • 阳性对照
    • Human placenta. CHO-cells transfected with CD163.
  • 常规说明


    ab17051 is very useful for macrophage phenotyping. Positive staining can be seen in the skin (histiocytes), gut, Kupffer cells, few aveolar macrophages, main population of macrophages in the placenta, varying degrees of macrophages in inflammed tissue including tumorous tissue depending on the inflammatory stage. Red pulp, not white pulp, macrophages in the spleen and cortical macrophages of the thymus are detected. Antibody is sensitive to ionic detergents and to chloroform/methanol treatment.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • 纯化说明0.2 µm filtered
  • Primary antibody说明ab17051 is very useful for macrophage phenotyping. Positive staining can be seen in the skin (histiocytes), gut, Kupffer cells, few aveolar macrophages, main population of macrophages in the placenta, varying degrees of macrophages in inflammed tissue including tumorous tissue depending on the inflammatory stage. Red pulp, not white pulp, macrophages in the spleen and cortical macrophages of the thymus are detected. Antibody is sensitive to ionic detergents and to chloroform/methanol treatment.
  • 克隆单克隆
  • 克隆编号RM3/1
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab17051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 130 kDa.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use a concentration of 4 µg/ml.
  • 应用说明Is unsuitable for IHC-G or IHC-P.
  • 靶标

    • 功能Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
      After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
    • 组织特异性Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.
    • 序列相似性Contains 9 SRCR domains.
    • 结构域The SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes.
    • 翻译后修饰A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
      Phosphorylated.
    • 细胞定位Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C163A_HUMAN antibody
      • CD 163 antibody
      • CD163 antibody
      • CD163 antigen antibody
      • CD163 molecule antibody
      • Hemoglobin scavenger receptor antibody
      • M130 antibody
      • M130 antigen precursor antibody
      • Macrophage associated antigen antibody
      • MM130 antibody
      • OTTHUMP00000238617 antibody
      • OTTHUMP00000238618 antibody
      • OTTHUMP00000238619 antibody
      • OTTHUMP00000238620 antibody
      • Scavenger receptor cysteine rich type 1 protein M130 antibody
      • sCD163 antibody
      • Soluble CD163 antibody
      see all

    Anti-CD163 antibody [RM3/1] (ab17051)参考文献

    This product has been referenced in:
    • Chamberlain CS  et al. Interleukin-1 receptor antagonist modulates inflammation and scarring after ligament injury. Connect Tissue Res 55:177-86 (2014). Read more (PubMed: 24649870) »
    • Chamberlain CS  et al. Interleukin expression after injury and the effects of interleukin-1 receptor antagonist. PLoS One 8:e71631 (2013). IHC-Fr ; Rat . Read more (PubMed: 23936523) »

    See all 8 Publications for this product

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    Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guaran...

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    Thank you for your enquiry and your interest in our products.

    Generally, each antibody and batch should be titrated to determine the optimal working dilutions/concentrations.

    ab17051: this antibody has only been tested in ICC/IF and...

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    Thank you for contacting us. The antibody ab17051 has been tested with rat samples and turned out to not be working for this species (indicated under "Does not react with"). The other antibodies for CD163 have not been tested with rat so...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Resin sections)
    Sample Human Tissue sections (Nasal polyp and tonsil)
    Specification Nasal polyp and tonsil
    Username

    Abcam user community

    Verified customer

    提交于 Sep 28 2010

    Thank you for getting back to me with further details. I appreciate the steps that you have taken to optimise this antibody and the control stainings that you detail. I am certainly prepared to offer you a credit note against this purchase provided ...

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    Thank you for your enquiry. I am sorry to the delay to your enquiry and sorry to hear that you have been having difficulties with this antibody. I have been in touch with the originator and they have offered the following suggestions. CD163 is a mem...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"