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CD163 antibody was raised against spleen cells from a case of hairy cell leukemia.
Our Abpromise guarantee covers the use of ab111250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 125 kDa.|
|IHC-P||Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||Use 0.5µg for 105-8 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
Negative control - immunohistochemical staining of paraffin-embedded formalin fixed human cerebral cortex using ab111250 at 20 µg/mL.
Immunohistochemical staining of paraffin-embedded formalin fixed human liver using ab111250 at 20 µg/mL.
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