Anti-CD162抗体[HECA-452] (ab110096)

概述

  • 产品名称Anti-CD162抗体[HECA-452]
    参阅全部 CD162 一抗
  • 描述
    大鼠单克隆抗体[HECA-452] to CD162
  • 经测试应用适用于: IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Raised against a stromal preparation of human tonsils

  • 阳性对照
    • Human spleen or tonsil tissue. Human peripheral blood lymphocytes.

性能

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应用

Our Abpromise guarantee covers the use of ab110096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab35768-Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.

靶标

  • 功能A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
    (Microbial infection) Acts as a receptor for enterovirus 71.
  • 组织特异性Expressed on neutrophils, monocytes and most lymphocytes.
  • 翻译后修饰Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
    Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 162 antibody
    • CD162 antibody
    • CD162 antigen antibody
    • CLA antibody
    • Cutaneous lymphocyte associated associated antigen antibody
    • P selectin glycoprotein ligand 1 antibody
    • P selectin glycoprotein ligand 1 precursor antibody
    • P-selectin glycoprotein ligand 1 antibody
    • PSGL 1 antibody
    • PSGL-1 antibody
    • PSGL1 antibody
    • Selectin P ligand antibody
    • SELPL_HUMAN antibody
    • SELPLG antibody
    see all

Anti-CD162 antibody [HECA-452] 图像

  • ab110096 at 10µg/ml staining CD162 in Formalin/PFA-fixed paraffin-embedded Human spleen tissue.
  • ab110096 at 10µg/ml staining CD162 in Formalin/PFA-fixed paraffin-embedded Human tonsil tissue.

Anti-CD162 antibody [HECA-452] (ab110096)参考文献

ab110096 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (LPS-inflamed lung)
Permeabilization No
Specification LPS-inflamed lung
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Formaldehyde
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提交于 Mar 07 2016

I am very pleased to hear you would like to accept our offer and test ab110096 in ICC/IF. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forICC/IF and include this code in the ...

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