概述

  • 产品名称Anti-CD162抗体[EPR1967Y]
    参阅全部 CD162 一抗
  • 描述
    兔单克隆抗体[EPR1967Y] to CD162
  • 经测试应用适用于: WB, IP, ICC, IHC-Gmore details
    不适用于: Flow Cyt or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in the extracellular region of CD162

  • 阳性对照
    • Jurkat cell lysate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab68143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 43 kDa).
IP 1/30.
ICC 1/100 - 1/250.
IHC-G Use at an assay dependent concentration. PubMed: 23635004
  • 应用说明Is unsuitable for Flow Cyt or IHC-P.
  • 靶标

    • 功能A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
      (Microbial infection) Acts as a receptor for enterovirus 71.
    • 组织特异性Expressed on neutrophils, monocytes and most lymphocytes.
    • 翻译后修饰Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
      Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD 162 antibody
      • CD162 antibody
      • CD162 antigen antibody
      • CLA antibody
      • Cutaneous lymphocyte associated associated antigen antibody
      • P selectin glycoprotein ligand 1 antibody
      • P selectin glycoprotein ligand 1 precursor antibody
      • P-selectin glycoprotein ligand 1 antibody
      • PSGL 1 antibody
      • PSGL-1 antibody
      • PSGL1 antibody
      • Selectin P ligand antibody
      • SELPL_HUMAN antibody
      • SELPLG antibody
      see all

    Anti-CD162 antibody [EPR1967Y] 图像

    • ab681432(Red) staining CD162 in Human bronchial tissue sections by Immunohistochemistry (Glycolmethacrylate sections). Tissue was embedded in Glycolmethacrylate and floated onto 0.2% ammonia solution prior to adherence to slides.Samples were incubated with primary antibody (1/200) for 1 hour. Samples were also stained with a neutrophil elastase antibody( Brown). Sections were counterstained with haematoxylin.

      Key: E + x200 magnification and F = x500 magnification. * = Double stained cells.

       

    • All lanes : Anti-CD162 antibody [EPR1967Y] (ab68143) at 1/500 dilution

      Lane 1 : Jurkat cell lysate at 10 µg
      Lane 2 : Recombinant CD162 protein at 5ng

      Secondary
      HRP conjugated Goat anti-rabit at 1/2000 dilution

      Predicted band size : 43 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)

    Anti-CD162 antibody [EPR1967Y] (ab68143)参考文献

    This product has been referenced in:
    • Blidberg K  et al. Adhesion molecules in subjects with COPD and healthy non-smokers: a cross sectional parallel group study. Respir Res 14:47 (2013). IHC-G ; Human . Read more (PubMed: 23635004) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab68143.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"