概述

  • 产品名称Anti-CD162抗体
    参阅全部 CD162 一抗
  • 描述
    兔多克隆抗体to CD162
  • 经测试应用适用于: IP, WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Cow, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human CD162.

    (Peptide available as ab73318.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: TE 671 Whole Cell HeLa Whole Cell Rat Thymus Tissue Lysate

性能

应用

Our Abpromise guarantee covers the use of ab66882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 43 kDa).

靶标

  • 功能A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
    (Microbial infection) Acts as a receptor for enterovirus 71.
  • 组织特异性Expressed on neutrophils, monocytes and most lymphocytes.
  • 翻译后修饰Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
    Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 162 antibody
    • CD162 antibody
    • CD162 antigen antibody
    • CLA antibody
    • Cutaneous lymphocyte associated associated antigen antibody
    • P selectin glycoprotein ligand 1 antibody
    • P selectin glycoprotein ligand 1 precursor antibody
    • P-selectin glycoprotein ligand 1 antibody
    • PSGL 1 antibody
    • PSGL-1 antibody
    • PSGL1 antibody
    • Selectin P ligand antibody
    • SELPL_HUMAN antibody
    • SELPLG antibody
    see all

Anti-CD162 antibody 图像

  • CD162 was immunoprecipitated using 0.5mg Rat thymus tissue extract, 5µg of Rabbit polyclonal to CD162 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Rat thymus tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab66882.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 105kDa; CD162

  • All lanes : Anti-CD162 antibody (ab66882) at 1 µg/ml

    Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Rat Thymus Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa. We are unsure as to the identity of these extra bands.
    CD162 contains a number of potential glycosylation and phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

Anti-CD162 antibody (ab66882)参考文献

ab66882 has not yet been referenced specifically in any publications.

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