Anti-CD161抗体[PK136] (Biotin) (ab22516)

概述

  • 产品名称Anti-CD161抗体[PK136] (Biotin)
    参阅全部 CD161 一抗
  • 描述
    小鼠单克隆抗体[PK136] to CD161 (Biotin)
  • 偶联物Biotin
  • 特异性This antibody recognises CD161c, and may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by ab22516 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Ab22516 is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes.
  • 经测试应用适用于: Functional Studies, Flow Cytmore details
  • 种属反应性
    与反应: Mouse
    不与反应: Rat, Human
  • 免疫原

    Tissue/ cell preparation (Mouse): Spleen and bone marrow cells from CE mice

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide.
    Constituents: 1% BSA, PBS, pH 7.4.
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明This antibody was been purified by affinity chromatography, from tissue culture supernatant.
  • 克隆单克隆
  • 克隆编号PK136
  • 骨髓瘤Sp2/0-Ag14
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab22516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration. In vivo depletion of NK cells and in vitro activation of NK cells.
Flow Cyt Use 10µl for 106 cells. ab97679-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.
  • 组织特异性Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype.
  • 序列相似性Contains 1 C-type lectin domain.
  • 翻译后修饰N-glycosylated. Contains sialic acid residues.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C-type lectin domain family 5 member B antibody
    • CD161 antibody
    • CLEC5B antibody
    • HNKR-P1a antibody
    • Killer Cell Lectin like Receptor Subfamily B Member 1 antibody
    • Killer cell lectin-like receptor subfamily B member 1 antibody
    • KLRB1 antibody
    • KLRB1_HUMAN antibody
    • Natural killer cell surface protein P1A antibody
    • NKR antibody
    • NKR P1 antibody
    • NKR-P1A antibody
    • NKRP1 antibody
    • NKRP1A antibody
    see all

Anti-CD161 antibody [PK136] (Biotin) (ab22516)参考文献

ab22516 has not yet been referenced specifically in any publications.

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