RabMAb

Anti-CD16抗体[EPR4333] (ab109223)

概述

  • 产品名称Anti-CD16抗体[EPR4333]
    参阅全部 CD16 一抗
  • 描述
    兔单克隆抗体[EPR4333] to CD16
  • 特异性ab109223 recognizes CD16a
  • 经测试应用适用于: ICC/IF, WBmore details
    不适用于: Flow Cyt,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CD16 aa 200 to the C-terminus. The exact sequence is proprietary.
    (Peptide available as ab202990)

  • 阳性对照
    • U937, THP-1, Jurkat, K562, C6, Raw264.7, and PC-12 cell lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:
    Anti-CD16 antibody (HRP) [EPR4333] (ab206073)

     

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

性能

应用

Our Abpromise guarantee covers the use of ab109223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
WB 1/50000 - 1/20000. Detects a band of approximately 45 kDa (predicted molecular weight: 29 kDa).Can be blocked with CD16 peptide (ab202990).
  • 应用说明Is unsuitable for Flow Cyt,IHC-P or IP.
  • 靶标

    • 功能Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
    • 组织特异性Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
    • 序列相似性Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • 翻译后修饰Glycosylated. Contains high mannose- and complex-type oligosaccharides.
      The soluble form is produced by a proteolytic cleavage.
    • 细胞定位Cell membrane. Secreted. Exists also as a soluble receptor.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD 16 antibody
      • CD 16a antibody
      • CD16 antibody
      • CD16a antibody
      • CD16a antigen antibody
      • CD16B antibody
      • CD16b antigen antibody
      • Fc fragment of IgG antibody
      • Fc fragment of IgG low affinity IIIa receptor (CD16) antibody
      • Fc fragment of IgG low affinity IIIa receptor antibody
      • Fc fragment of IgG receptor IIIa antibody
      • Fc fragment of IgG, low affinity III, receptor (CD16) antibody
      • Fc fragment of IgG, low affinity III, receptor for (CD16) antibody
      • Fc fragment of IgG, low affinity IIIa, receptor (CD16) antibody
      • Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antibody
      • Fc fragment of IgG, low affinity IIIa, receptor for antibody
      • Fc fragment of IgG, low affinity IIIb, receptor (CD16b) antibody
      • Fc fragment of IgG, low affinity IIIb, receptor for (CD16) antibody
      • Fc gamma R3 antibody
      • Fc gamma receptor III 2 (CD 16) antibody
      • Fc gamma receptor III A antibody
      • Fc gamma receptor IIIA antibody
      • Fc gamma receptor IIIb (CD 16) antibody
      • Fc gamma RIII alpha antibody
      • Fc gamma RIII antibody
      • Fc gamma RIII beta antibody
      • Fc gamma RIIIa antibody
      • Fc gamma RIIIb antibody
      • Fc of IgG antibody
      • Fc-gamma receptor III2 (CD 16) antibody
      • Fc-gamma receptor III2 (CD16) antibody
      • Fc-gamma receptor IIIb (CD16) antibody
      • Fc-gamma RIII antibody
      • Fc-gamma RIII-alpha antibody
      • Fc-gamma RIIIa antibody
      • FCG 3 antibody
      • FCG3 antibody
      • FCG3A_HUMAN antibody
      • FCgammaRIIIA antibody
      • FCGR 3 antibody
      • FCGR 3A antibody
      • FCGR3 antibody
      • FCGR3A antibody
      • FCGR3A protein antibody
      • FCGRIII antibody
      • FCGRIII-2 antibody
      • FcR 10 antibody
      • FcR-10 antibody
      • FcR10 antibody
      • FcRIII antibody
      • FcRIIIa antibody
      • IGFR 3 antibody
      • IGFR3 antibody
      • IgG Fc receptor III 1 antibody
      • IgG Fc receptor III 2 antibody
      • IgG Fc receptor III-2 antibody
      • IMD20 antibody
      • immunoglobulin G Fc receptor III antibody
      • immunoglobulin G Fc receptor III-2 antibody
      • Low affinity IIIa receptor antibody
      • Low affinity immunoglobulin gamma Fc region receptor III A antibody
      • Low affinity immunoglobulin gamma Fc region receptor III-A antibody
      • Low affinity immunoglobulin gamma Fc region receptor IIIB antibody
      • neutrophil-specific antigen NA antibody
      see all

    Anti-CD16 antibody [EPR4333] 图像

    • Immunofluorescence staining of U937 cells with purified ab109223 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

    • All lanes : Anti-CD16 antibody [EPR4333] (ab109223) at 1/50000 dilution

      Lane 1 : U937 cell lysate
      Lane 2 : THP-1 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : K562 cell lysate
      Lane 5 : C6 cell lysate
      Lane 6 : Raw264.7 cell lysate
      Lane 7 : PC-12 cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 29 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)

    Anti-CD16 antibody [EPR4333] (ab109223)参考文献

    ab109223 has not yet been referenced specifically in any publications.

    Product Wall

    The amino acid sequence for ab109223 anti-CD16 antibody is considered to be commercially sensitive. However I may tell you that the immunogen is located in the cytoplasmic region of this antigen (http://www.uniprot.org/uniprot/P08637) To our knowled...

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    Thank you for your patience.

    For ab109223, based on the database and sequence information I received from the lab, this antibody would theoretically also cross react with CD16b.

    I hope this information is helpful to you. Please do...

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    Thank you for your patience.

    For ab109223, the lab let me know that this antibody is not predicted to cross-react with CD16b. The exact immunogen does not share any significant sequence similarity with CD16b.

    However, based on the ...

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    Thank you for contacting us.

    I have contacted the lab regarding ab109223, and will let you know what I hear back.

    And I have checked the homology of human CD16a with human CD16b for the following antibodies we had discussed:
    <...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"