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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CD16 aa 200 to the C-terminus. The exact sequence is proprietary.
(Peptide available as
Our Abpromise guarantee covers the use of ab109223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/50000 - 1/20000. Detects a band of approximately 45 kDa (predicted molecular weight: 29 kDa).Can be blocked with CD16 peptide (ab202990).|
Immunofluorescence staining of U937 cells with purified ab109223 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.
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