Anti-CD151抗体(ab125363)

概述

  • 产品名称Anti-CD151抗体
    参阅全部 CD151 一抗
  • 描述
    兔多克隆抗体to CD151
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig, African Green Monkey
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 154-203 (HCCGSNNSQD WRDSEWIRSQ EAGGRVVPDS CCKTVVALCG QRDHASNIYK) of Human CD151 (NP_620599).

  • 阳性对照
    • A549 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab125363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Essential for the proper assembly of the glomerular and tubular basement membranes in kidney.
  • 组织特异性Expressed in a variety of tissues including vascular endothelium and epidermis. Expressed on erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes.
  • 疾病相关Defects in CD151 are the cause of nephropathy with pretibial epidermolysis bullosa and deafness (NPEBD) [MIM:609057]. NPEBD is characterized by the association of hereditary nephritis, epidermolysis bullosa, deafness, and beta-thalassemia minor.
  • 序列相似性Belongs to the tetraspanin (TM4SF) family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD151 antibody
    • CD151 antigen antibody
    • CD151 molecule (Raph blood group) antibody
    • CD151 molecule antibody
    • CD151_HUMAN antibody
    • GP27 antibody
    • hemidesmosomal tetraspanin CD151 antibody
    • Membrane glycoprotein SFA-1 antibody
    • MER2 antibody
    • PETA-3 antibody
    • PETA3 antibody
    • Platelet endothelial tetraspan antigen 3 antibody
    • platelet surface glycoprotein gp27 antibody
    • Platelet-endothelial cell tetraspan antigen 3 antibody
    • Platelet-endothelial tetraspan antigen 3 antibody
    • RAPH antibody
    • Red blood cell antigen MER2 antibody
    • SFA 1 antibody
    • SFA1 antibody
    • Tetraspanin-24 antibody
    • Tspan-24 antibody
    • TSPAN24 antibody
    see all

Anti-CD151 antibody 图像

  • Species+tissue/cell type:Human nasal epithelial cells. Primary antibody dilution: 1:1000. Secondary antibody: Goat anti-rabbit Alexa Fluor 546. Secondary antibody dilution:1:1000

  • Anti-CD151 antibody (ab125363) at 1 µg/ml + A549 cell lysate at 10 µg

    Predicted band size : 28 kDa
    Gel concentration: 12%

Anti-CD151 antibody (ab125363)参考文献

ab125363 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: tris-HCl EDTA buffer
Permeabilization Yes - H2O2, TBS, Tween
Specification skin
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Lenka Sucha

Verified customer

提交于 Jun 16 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"