Reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. We have data to indicate that this antibody may not cross react with Pig. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 10µl for 106 cells. ab154431-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis.
Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi.