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Our Abpromise guarantee covers the use of ab34016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/25 - 1/50.
Use 10µl of the suggested working dilution to label 1 x 106 cells. Note that for staining of whole blood samples increased amounts of antibody may be required due to the presence of CD147 on erythrocytes.
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.|
WB using ab34016.
U: Untransfected HEK-293T cell lysate.
2d: HEK-293T cells transfected with mouse CD147 d1+2 construct.
3d: HEK-293T cells transfected with mouse CD147d0-2 construct.
Data from PMID : 14606962.
Copyright © 2003 Hanna et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"