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产品名称
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描述
小鼠单克隆抗体[P1H12] to CD146 (FITC)
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宿主
Mouse
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偶联物
FITC. Ex: 493nm, Em: 528nm
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特异性
ab78451 reacts specifically with CD146.
In blood and marrow ab78451 reacts only with endothelial cells, and has been used to detect circulating endothelial cells in human, dogs, rabbits and mouse samples.
ab78451 positively stains normal, primary HUVEC and MVEC cultures and the endothelial cells of all vessels in normal frozen sections of human skin, intestine, ovary tonsil, lymph node, lung, and kidney. It does not stain carcinoma cell lines HT-29 and COLO205, and M21, the T cell lines Jurkat and HuT78, fibroblasts, HL-60 or CHO cells, EBV-transformed B cell lines, normal monocytes, granulocytes, red cells, platelets, T cells or B cells from marrow or peripheral blood; nor does it detect marrow megakaryocytes or the megakaryoblast line HU3. The peripheral blood cells that do stain with ab78451 are also positive for both von Willebrand Factor (vWF) and thrombomodulin (the combined expression of which is limited to endothelium), and they stain for flt and flk. In recent testing, melanoma A2058, SKMEL.3 and A375.S2 are positive, while murine melanoma M3 is negative. One smooth muscle line (HISM) was positive and one negative (TIGHA-VSMC). Human blood lymphoctes are negative with or without prior stimulation. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
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经测试应用
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种属反应性
与反应:
Mouse, Dog, Human
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免疫原
Immunization of mice with HUVEC
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阳性对照
- Human, mouse or dog blood or marrow cells
Human intestinal smooth muscle cells
Melanoma A2058, SKMEL.3 or A375.S2 cells
Normal, primary HUVEC or MVEC cultures
Human skin, intestine, ovary tonsil, lymph node, lung or kidney tissue.