Anti-CD146抗体(ab203118)
Key features and details
- Rabbit polyclonal to CD146
- Suitable for: IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-CD146抗体
参阅全部 CD146 一抗 -
描述
兔多克隆抗体to CD146 -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide within Human CD146 aa 200-300 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P43121 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab203118于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 1/50 - 1/200 with fluorescent detection methods. |
说明 |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use at 1/50 - 1/200 with fluorescent detection methods. |
靶标
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功能
Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration. -
组织特异性
Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis. -
序列相似性
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4162 Human
- Entrez Gene: 78967 Rat
- Omim: 155735 Human
- SwissProt: P43121 Human
- SwissProt: Q9EPF2 Rat
- Unigene: 599039 Human
- Unigene: 2694 Rat
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别名
- A32 antigen antibody
- CD 146 antibody
- CD146 antibody
see all
图片
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Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat brain tissue sections labeling CD146 with ab203118 at a 1/400 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human cervical carcinoma tissue sections labeling CD146 with ab203118 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab203118 被引用在 1 文献中.
- Wang X et al. Influence of the intensity and loading time of direct current electric field on the directional migration of rat bone marrow mesenchymal stem cells. Front Med N/A:N/A (2016). PubMed: 27324024