概述

  • 产品名称Anti-CD14抗体[Tuk4]
    参阅全部 CD14 一抗
  • 描述
    小鼠单克隆抗体[Tuk4] to CD14
  • 特异性Ab27545 recognises CD14 surface antigen, expressed strongly by most peripheral blood monocytes and weakly on granulocytes.
  • 经测试应用适用于: WB, ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Sheep, Rabbit, Goat, Cow, Cat, Dog, Human, Pig, Mink (Mustela), Cynomolgus Monkey
  • 常规说明

    Clone Tuk4 has been shown to block SDF induced chemotaxis of U937 cells in a dose dependent manner. This antibody could therefore be recommended for this purpose.

性能

应用

Our Abpromise guarantee covers the use of ab27545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.

Use under reducing conditions.

ELISA Use at an assay dependent concentration.
Flow Cyt 1/50 - 1/200.

Use 5µl of the suggested working dilution to label 106 cells or 100µl whole blood.





ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • 组织特异性Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • 序列相似性Contains 11 LRR (leucine-rich) repeats.
  • 翻译后修饰N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 14 antibody
    • CD_antigen=CD14 antibody
    • CD14 antibody
    • CD14 antigen antibody
    • CD14 molecule antibody
    • CD14_HUMAN antibody
    • LPS-R antibody
    • Mo2 antibody
    • Monocyte differentiation antigen CD14 antibody
    • Monocyte differentiation antigen CD14 urinary form antibody
    • Monocyte differentiation antigen CD14, membrane-bound form antibody
    • Myeloid cell specific leucine rich glycoprotein antibody
    • Myeloid cell-specific leucine-rich glycoprotein antibody
    see all

Anti-CD14 antibody [Tuk4] (ab27545)参考文献

This product has been referenced in:
  • Slobedman B  et al. Latent cytomegalovirus down-regulates major histocompatibility complex class II expression on myeloid progenitors. Blood 100:2867-73 (2002). Read more (PubMed: 12351397) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (normal dog spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid / Tris EDTA
Permeabilization No
Specification normal dog spleen
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Formaldehyde
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提交于 May 18 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"