Anti-CD14抗体[MEM-15] (FITC) (ab28061)

概述

  • 产品名称
    Anti-CD14抗体[MEM-15] (FITC)
    参阅全部 CD14 一抗
  • 描述
    小鼠单克隆抗体[MEM-15] to CD14 (FITC)
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm
  • 特异性
    Ab28061 recognises CD14 expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. Ab28061 also recognises soluble forms of CD14 found in serum and in the urine of some nephrotic patients.
  • 经测试应用
    适用于: ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified): A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.

  • 常规说明

    The antibody is free of unconjugated FITC.

性能

应用

Our Abpromise guarantee covers the use of ab28061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 20µl for 106 cells.

or 100 µl of whole blood.

靶标

  • 功能
    Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • 组织特异性
    Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • 序列相似性
    Contains 11 LRR (leucine-rich) repeats.
  • 翻译后修饰
    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 14 antibody
    • CD_antigen=CD14 antibody
    • CD14 antibody
    • CD14 antigen antibody
    • CD14 molecule antibody
    • CD14_HUMAN antibody
    • LPS-R antibody
    • Mo2 antibody
    • Monocyte differentiation antigen CD14 antibody
    • Monocyte differentiation antigen CD14 urinary form antibody
    • Monocyte differentiation antigen CD14, membrane-bound form antibody
    • Myeloid cell specific leucine rich glycoprotein antibody
    • Myeloid cell-specific leucine-rich glycoprotein antibody
    see all

图片

  • Flow cytometry analysis of human peripheral blood cells labelling CD14 with ab28061.

  • ab28061 staining CD14 in human peripheral blood monocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 2% paraformaldehyde for 20 minutes at room temperature and blocked with 2% goat serum for 15 minutes at 25°C. Samples were incubated with the undiluted primary antibody for 45 minutes at 25°C.

    See Abreview

  • ab28061 staining CD14 in peripheral blood mononuclear cells by flow cytometry. The fibroblasts were prepared using 10 uL FITC antiCD14 to 50 uL (~100,000) cells in PBS/5%FCS and incubated on ice for 45 min. Cells were gated on viable cells.

    See Abreview

文献

This product has been referenced in:
  • Gonda K  et al. Myeloid-derived suppressor cells are increased and correlated with type 2 immune responses, malnutrition, inflammation, and poor prognosis in patients with breast cancer. Oncol Lett 14:1766-1774 (2017). Read more (PubMed: 28789407) »
  • Steensma MR  et al. Targeting the giant cell tumor stromal cell: functional characterization and a novel therapeutic strategy. PLoS One 8:e69101 (2013). Flow Cyt ; Human . Read more (PubMed: 23922683) »

See all 3 Publications for this product

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: 25°C
Sample
Human Cell (Peripheral Blood Monocyte)
Specification
Peripheral Blood Monocyte
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 15 2014

Thank you for your inquiry. We do not recommend to freeze this antibody and to especially avoid freeze/thaw cycles. But I would like to recommend to run a test experiment. Antibodies in general are very stable and as long as the conjuga...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Peripheral Blood Mononuclear Cell)
Specification
Peripheral Blood Mononuclear Cell
Fixation
none
Permeabilization
No
Gating Strategy
monocytes
Username

Abcam user community

Verified customer

提交于 Oct 20 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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