概述

  • 产品名称Anti-CD14抗体
    参阅全部 CD14 一抗
  • 描述
    山羊多克隆抗体to CD14
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    C-KRVDADADPRQYAD

    , corresponding to Internal sequence amino acids 71-84 of Human CD14

  • 阳性对照
    • Human Lymph Node lysate and tonsil lysates. Human spleen tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab45870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 40 kDa). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun. 2001 Jun;69(6):3772-81; PMID: 11349042).
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

靶标

  • 功能Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • 组织特异性Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • 序列相似性Contains 11 LRR (leucine-rich) repeats.
  • 翻译后修饰N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 14 antibody
    • CD_antigen=CD14 antibody
    • CD14 antibody
    • CD14 antigen antibody
    • CD14 molecule antibody
    • CD14_HUMAN antibody
    • LPS-R antibody
    • Mo2 antibody
    • Monocyte differentiation antigen CD14 antibody
    • Monocyte differentiation antigen CD14 urinary form antibody
    • Monocyte differentiation antigen CD14, membrane-bound form antibody
    • Myeloid cell specific leucine rich glycoprotein antibody
    • Myeloid cell-specific leucine-rich glycoprotein antibody
    see all

Anti-CD14 antibody 图像

  • Anti-CD14 antibody (ab45870) at 1 µg/ml + Human Lymph Node lysate (35µg protein in RIPA buffer).

    Predicted band size : 40 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • ab45870 at 4µg/ml staining CD14 in human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    Image shows strong clustered staining in some splenocytes.

Anti-CD14 antibody (ab45870)参考文献

This product has been referenced in:
  • Li Y & Ding J Optimized generation of survivin-specific cytotoxic T lymphocytes against lung cancer. Mol Med Rep 12:2169-74 (2015). Read more (PubMed: 25845433) »
  • Azakir BA  et al. Proteasome inhibitors increase missense mutated dysferlin in patients with muscular dystrophy. Sci Transl Med 6:250ra112 (2014). Human . Read more (PubMed: 25143362) »

See all 5 Publications for this product

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with product ab45870. To check the status of the order please contact our Cust...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"