Anti-CD14抗体[61D3] (PE/Cy5.5 ®) (ab25390)

概述

  • 产品名称Anti-CD14抗体[61D3] (PE/Cy5.5 ®)
    参阅全部 CD14 一抗
  • 描述
    小鼠单克隆抗体[61D3] to CD14 (PE/Cy5.5 ®)
  • 偶联物PE/Cy5.5 ®. Ex: 496nm, Em: 690nm
  • 特异性Human CD14
  • 经测试应用适用于: Flow Cyt, IHC-Fr, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 常规说明This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

性能

应用

Our Abpromise guarantee covers the use of ab25390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 10µl for 106 cells. ab154453-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

靶标

  • 功能Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • 组织特异性Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • 序列相似性Contains 11 LRR (leucine-rich) repeats.
  • 翻译后修饰N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 14 antibody
    • CD_antigen=CD14 antibody
    • CD14 antibody
    • CD14 antigen antibody
    • CD14 molecule antibody
    • CD14_HUMAN antibody
    • LPS-R antibody
    • Mo2 antibody
    • Monocyte differentiation antigen CD14 antibody
    • Monocyte differentiation antigen CD14 urinary form antibody
    • Monocyte differentiation antigen CD14, membrane-bound form antibody
    • Myeloid cell specific leucine rich glycoprotein antibody
    • Myeloid cell-specific leucine-rich glycoprotein antibody
    see all

Anti-CD14 antibody [61D3] (PE/Cy5.5 ®) (ab25390)参考文献

This product has been referenced in:
  • Mund JA  et al. Human proangiogenic circulating hematopoietic stem and progenitor cells promote tumor growth in an orthotopic melanoma xenograft model. Angiogenesis 16:953-62 (2013). Read more (PubMed: 23877751) »
  • Lobba AR  et al. Differential expression of CD90 and CD14 stem cell markers in malignant breast cancer cell lines. Cytometry A 81:1084-91 (2012). Flow Cyt . Read more (PubMed: 23090904) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: 25°C
Sample Human Cell (Peripheral Blood Mononuclear Cells (PBMC))
Specification Peripheral Blood Mononuclear Cells (PBMC)
Permeabilization No
Fixative Paraformaldehyde
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提交于 Oct 22 2014

Thank you for your inquiry. The isotype of ab25390 is Mouse IgG1k. I hope this information helps. Please let us know if you have any other questions or require additional information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"