Anti-CD14抗体[47-3D6] (Allophycocyanin) (ab91144)

概述

  • 产品名称
    Anti-CD14抗体[47-3D6] (Allophycocyanin)
    参阅全部 CD14 一抗
  • 描述
    小鼠单克隆抗体[47-3D6] to CD14 (Allophycocyanin)
  • 偶联物
    Allophycocyanin. Ex: 645nm, Em: 660nm
  • 特异性
    ab91144 reacts with Human monocytes and macrophages; weak reactions may occur with neutrophils.
  • 经测试应用
    适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human lung angiotensin converting enzyme.

  • 阳性对照
    • Human normal peripheral blood.

性能

应用

Our Abpromise guarantee covers the use of ab91144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明
    Flow Cyt: Use 20µl for 106 cells.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
    • 组织特异性
      Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
    • 序列相似性
      Contains 11 LRR (leucine-rich) repeats.
    • 翻译后修饰
      N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
    • 细胞定位
      Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD 14 antibody
      • CD_antigen=CD14 antibody
      • CD14 antibody
      • CD14 antigen antibody
      • CD14 molecule antibody
      • CD14_HUMAN antibody
      • LPS-R antibody
      • Mo2 antibody
      • Monocyte differentiation antigen CD14 antibody
      • Monocyte differentiation antigen CD14 urinary form antibody
      • Monocyte differentiation antigen CD14, membrane-bound form antibody
      • Myeloid cell specific leucine rich glycoprotein antibody
      • Myeloid cell-specific leucine-rich glycoprotein antibody
      see all

    图片

    • ab91144, at 20µl/106 cells, staining CD14 in normal Human peripheral blood by Flow Cytometry.
    • ab91144, at 20µl/106 cells, staining CD14 in normal Human peripheral blood by Flow Cytometry.

    文献

    ab91144 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Human Cell (PBMC)
    Specification
    PBMC
    Preparation
    Cell harvesting/tissue preparation method: Ficoll-Hypaque density gradient
    Sample buffer: PBS
    Permeabilization
    No
    Gating Strategy
    monocytes
    Username

    Abcam user community

    Verified customer

    提交于 Apr 10 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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